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PMID:20859253

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Citation

Kamminga, LM, Luteijn, MJ, den Broeder, MJ, Redl, S, Kaaij, LJ, Roovers, EF, Ladurner, P, Berezikov, E and Ketting, RF (2010) Hen1 is required for oocyte development and piRNA stability in zebrafish. EMBO J. 29:3688-700

Abstract

Piwi-interacting RNAs (piRNAs) are germ line-specific small RNA molecules that have a function in genome defence and germ cell development. They associate with a specific class of Argonaute proteins, named Piwi, and function through an RNA interference-like mechanism. piRNAs carry a 2'-O-methyl modification at their 3' end, which is added by the Hen1 enzyme. We show that zebrafish hen1 is specifically expressed in germ cells and is essential for maintaining a female germ line, whereas it is dispensable in the testis. Hen1 protein localizes to nuage through its C-terminal domain, but is not required for nuage formation. In hen1 mutant testes, piRNAs become uridylated and adenylated. Uridylation frequency is highest on retro-transposon-derived piRNAs and is accompanied by decreased piRNA levels and mild derepression of transposon transcripts. Altogether, our data suggest the existence of a uridylation-mediated 3'-5' exonuclease activity acting on piRNAs in zebrafish germ cells, which is counteracted by nuage-bound Hen1 protein. This system discriminates between piRNA targets and is required for ovary development and fully efficient transposon silencing.

Links

PubMed PMC2982757 Online version:10.1038/emboj.2010.233

Keywords

Amino Acid Sequence; Animals; Blotting, Northern; Embryo, Nonmammalian/cytology; Embryo, Nonmammalian/metabolism; Female; Immunoprecipitation; In Situ Hybridization; Male; Methyltransferases/metabolism; Methyltransferases/physiology; Molecular Sequence Data; Mutation/genetics; Oocytes/cytology; Oocytes/metabolism; RNA 3' End Processing/physiology; RNA, Messenger/genetics; RNA, Small Interfering/chemistry; RNA, Small Interfering/genetics; RNA, Small Interfering/metabolism; Retroelements; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid; Subcellular Fractions; Testis/cytology; Testis/metabolism; Uridine/metabolism; Zebrafish/genetics; Zebrafish/growth & development; Zebrafish/metabolism; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism; Zebrafish Proteins/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:HENMT

enables

GO:0008171: O-methyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:HENMT

enables

GO:0008173: RNA methyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:HENMT

involved_in

GO:0001510: RNA methylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:HENMT

located_in

GO:0043186: P granule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:HENMT

involved_in

GO:0048599: oocyte development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-101118-2

P

Seeded From UniProt

complete

DANRE:HENMT

involved_in

GO:0034587: piRNA metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-101118-2

P

Seeded From UniProt

complete

DANRE:PIWL1

located_in

GO:0043186: P granule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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