PMID:20838898

Citation	Bakhrat, A, Pritchett, T, Peretz, G, McCall, K and Abdu, U (2010) Drosophila Chk2 and p53 proteins induce stage-specific cell death independently during oogenesis. Apoptosis 15:1425-34
Abstract	In Drosophila, the checkpoint protein-2 kinase (DmChk2) and its downstream effector protein, Dmp53, are required for DNA damage-mediated cell cycle arrest, DNA repair and apoptosis. In this study we focus on understanding the function of these two apoptosis inducing factors during ovarian development. We found that expression of Dmp53, but not DmChk2, led to loss of ovarian stem cells. We demonstrate that expression of DmChk2, but not Dmp53, induced mid-oogenesis cell death. DmChk2 induced cell death was not suppressed by Dmp53 mutant, revealing for the first time that in Drosophila, over-expression of DmChk2 can induce cell death which is independent of Dmp53. We found that over-expression of caspase inhibitors such as DIAP1, p35 and p49 did not suppress DmChk2- and Dmp53-induced cell death. Thus, our study reveals stage-specific effects of Dmp53 and DmChk2 in oogenesis. Moreover, our results demonstrate that although DmChk2 and Dmp53 affect different stages of ovarian development, loss of ovarian stem cells by p53 expression and mid-oogenesis cell death induced by DmChk2 do not require caspase activity.
Links	PubMed PMC3047510 Online version:10.1007/s10495-010-0539-z
Keywords	Animals; Animals, Genetically Modified; Apoptosis/genetics; Autophagy/genetics; Caspases/antagonists & inhibitors; Caspases/metabolism; Cloning, Molecular; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/physiology; Female; Genes, Insect; Inhibitor of Apoptosis Proteins/genetics; Inhibitor of Apoptosis Proteins/metabolism; Oogenesis/genetics; Ovarian Follicle/physiology; Ovarian Follicle/ultrastructure; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Stem Cells/cytology; Stem Cells/metabolism; Tumor Suppressor Protein p53/genetics; Tumor Suppressor Protein p53/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
DROME:A0A0B4K7P1	involved_in	GO:0035234: ectopic germ cell programmed cell death	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:LOK	involved_in	GO:0035234: ectopic germ cell programmed cell death	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:Q2XVY6	involved_in	GO:0035234: ectopic germ cell programmed cell death	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:Q2XVY7	involved_in	GO:0035234: ectopic germ cell programmed cell death	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:Q8IH92	involved_in	GO:0035234: ectopic germ cell programmed cell death	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:Q9N6D8	involved_in	GO:0035234: ectopic germ cell programmed cell death	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:Q8IMZ4	involved_in	GO:0035234: ectopic germ cell programmed cell death	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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