GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:20829360

From GONUTS
Jump to: navigation, search
Citation

Carrie, C, Giraud, E, Duncan, O, Xu, L, Wang, Y, Huang, S, Clifton, R, Murcha, M, Filipovska, A, Rackham, O, Vrielink, A and Whelan, J (2010) Conserved and novel functions for Arabidopsis thaliana MIA40 in assembly of proteins in mitochondria and peroxisomes. J. Biol. Chem. 285:36138-48

Abstract

The disulfide relay system of the mitochondrial intermembrane space has been extensively characterized in Saccharomyces cerevisiae. It contains two essential components, Mia40 and Erv1. The genome of Arabidopsis thaliana contains a single gene for each of these components. Although insertional inactivation of Erv1 leads to a lethal phenotype, inactivation of Mia40 results in no detectable deleterious phenotype. A. thaliana Mia40 is targeted to and accumulates in mitochondria and peroxisomes. Inactivation of Mia40 results in an alteration of several proteins in mitochondria, an absence of copper/zinc superoxide dismutase (CSD1), the chaperone for superoxide dismutase (Ccs1) that inserts copper into CSD1, and a decrease in capacity and amount of complex I. In peroxisomes the absence of Mia40 leads to an absence of CSD3 and a decrease in abnormal inflorescence meristem 1 (Aim1), a β-oxidation pathway enzyme. Inactivation of Mia40 leads to an alteration of the transcriptome of A. thaliana, with genes encoding peroxisomal proteins, redox functions, and biotic stress significantly changing in abundance. Thus, the mechanistic operation of the mitochondrial disulfide relay system is different in A. thaliana compared with other systems, and Mia40 has taken on new roles in peroxisomes and mitochondria.

Links

PubMed PMC2975236 Online version:10.1074/jbc.M110.121202

Keywords

Amino Acid Sequence; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/classification; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Arabidopsis Proteins/physiology; Blotting, Western; Conserved Sequence; DNA, Bacterial/genetics; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes, Essential/genetics; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Mitochondria/metabolism; Mitochondrial Proteins/genetics; Mitochondrial Proteins/metabolism; Mitochondrial Proteins/physiology; Molecular Sequence Data; Multienzyme Complexes/genetics; Multienzyme Complexes/metabolism; Mutagenesis, Insertional; Mutation; Oligonucleotide Array Sequence Analysis; Peroxisomes/metabolism; Phylogeny; Protein Transport; Sequence Homology, Amino Acid; Superoxide Dismutase/genetics; Superoxide Dismutase/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:MIA40

acts_upstream_of_or_within

GO:0006626: protein targeting to mitochondrion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:MIA40

acts_upstream_of_or_within

GO:0006625: protein targeting to peroxisome

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:MIA40

located_in

GO:0005739: mitochondrion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:MIA40

located_in

GO:0005777: peroxisome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.