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PMID:20812917

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Citation

Watanabe, Y, Takeuchi, K, Higa Onaga, S, Sato, M, Tsujita, M, Abe, M, Natsume, R, Li, M, Furuichi, T, Saeki, M, Izumikawa, T, Hasegawa, A, Yokoyama, M, Ikegawa, S, Sakimura, K, Amizuka, N, Kitagawa, H and Igarashi, M (2010) Chondroitin sulfate N-acetylgalactosaminyltransferase-1 is required for normal cartilage development. Biochem. J. 432:47-55

Abstract

CS (chondroitin sulfate) is a glycosaminoglycan species that is widely distributed in the extracellular matrix. To understand the physiological roles of enzymes involved in CS synthesis, we produced CSGalNAcT1 (CS N-acetylgalactosaminyltransferase 1)-null mice. CS production was reduced by approximately half in CSGalNAcT1-null mice, and the amount of short-chain CS was also reduced. Moreover, the cartilage of the null mice was significantly smaller than that of wild-type mice. Additionally, type-II collagen fibres in developing cartilage were abnormally aggregated and disarranged in the homozygous mutant mice. These results suggest that CSGalNAcT1 is required for normal CS production in developing cartilage.

Links

PubMed PMC2995422 Online version:10.1042/BJ20100847

Keywords

Animals; Blotting, Western; Cell Line; Cell Proliferation; Chondrocytes/cytology; Chondrocytes/enzymology; Chondrocytes/metabolism; Chondrogenesis; Chondroitin Sulfates/biosynthesis; Collagen Type II/metabolism; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Growth Plate/embryology; Growth Plate/enzymology; Growth Plate/metabolism; Immunohistochemistry; Mice; Mice, Inbred C57BL; Mice, Knockout; N-Acetylgalactosaminyltransferases/genetics; N-Acetylgalactosaminyltransferases/metabolism; Proliferating Cell Nuclear Antigen/metabolism; Reverse Transcriptase Polymerase Chain Reaction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:CO2A1

part_of

GO:0005581: collagen trimer

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:35505)

Seeded From UniProt

complete

MOUSE:COAA1

part_of

GO:0005581: collagen trimer

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:35506)|part_of:(EMAPA:19143)

Seeded From UniProt

complete

MOUSE:CGAT1

acts_upstream_of_or_within

GO:0030204: chondroitin sulfate metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:4868209

P

Seeded From UniProt

complete

MOUSE:CGAT1

acts_upstream_of_or_within

GO:0051216: cartilage development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:4868209

P

  • results_in_development_of:(EMAPA:32730)

Seeded From UniProt

complete


See also

References

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