GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:20807199

From GONUTS
Jump to: navigation, search
Citation

Davies, BW, Köhrer, C, Jacob, AI, Simmons, LA, Zhu, J, Aleman, LM, Rajbhandary, UL and Walker, GC (2010) Role of Escherichia coli YbeY, a highly conserved protein, in rRNA processing. Mol. Microbiol. 78:506-18

Abstract

The UPF0054 protein family is highly conserved with homologues present in nearly every sequenced bacterium. In some bacteria, the respective gene is essential, while in others its loss results in a highly pleiotropic phenotype. Despite detailed structural studies, a cellular role for this protein family has remained unknown. We report here that deletion of the Escherichia coli homologue, YbeY, causes striking defects that affect ribosome activity, translational fidelity and ribosome assembly. Mapping of 16S, 23S and 5S rRNA termini reveals that YbeY influences the maturation of all three rRNAs, with a particularly strong effect on maturation at both the 5'- and 3'-ends of 16S rRNA as well as maturation of the 5'-termini of 23S and 5S rRNAs. Furthermore, we demonstrate strong genetic interactions between ybeY and rnc (encoding RNase III), ybeY and rnr (encoding RNase R), and ybeY and pnp (encoding PNPase), further suggesting a role for YbeY in rRNA maturation. Mutation of highly conserved amino acids in YbeY, allowed the identification of two residues (H114, R59) that were found to have a significant effect in vivo. We discuss the implications of these findings for rRNA maturation and ribosome assembly in bacteria.

Links

PubMed PMC2959132 Online version:10.1111/j.1365-2958.2010.07351.x

Keywords

Amino Acid Sequence; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Deletion; Metalloproteins/genetics; Metalloproteins/metabolism; Molecular Sequence Data; Prokaryotic Initiation Factors/metabolism; Protein Binding; RNA, Bacterial/metabolism; RNA, Ribosomal/metabolism; Ribosomes/metabolism; Sequence Alignment

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:YBEY

involved_in

GO:0006364: rRNA processing

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:YBEY

involved_in

GO:0042254: ribosome biogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:YBEY

acts_upstream_of_or_within

GO:0000478: endonucleolytic cleavage involved in rRNA processing

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:YBEY

acts_upstream_of_or_within

GO:0030490: maturation of SSU-rRNA

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOBW:RRMF

GO:2000234: positive regulation of rRNA processing

ECO:0000270:

P

Fig. 3

complete
CACAO 3861


See also

References

See Help:References for how to manage references in GONUTS.