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PMID:20802481

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Citation

Jiao, X, Xiang, S, Oh, C, Martin, CE, Tong, L and Kiledjian, M (2010) Identification of a quality-control mechanism for mRNA 5'-end capping. Nature 467:608-11

Abstract

The 7-methylguanosine cap structure at the 5' end of eukaryotic messenger RNAs is a critical determinant of their stability and translational efficiency. It is generally believed that 5'-end capping is a constitutive process that occurs during mRNA maturation and lacks the need for a quality-control mechanism to ensure its fidelity. We recently reported that the yeast Rai1 protein has pyrophosphohydrolase activity towards mRNAs lacking a 5'-end cap. Here we show that, in vitro as well as in yeast cells, Rai1 possesses a novel decapping endonuclease activity that can also remove the entire cap structure dinucleotide from an mRNA. This activity is targeted preferentially towards mRNAs with unmethylated caps in contrast to the canonical decapping enzyme, Dcp2, which targets mRNAs with a methylated cap. Capped but unmethylated mRNAs generated in yeast cells with a defect in the methyltransferase gene are more stable in a rai1-gene-disrupted background. Moreover, rai1Δ yeast cells with wild-type capping enzymes show significant accumulation of mRNAs with 5'-end capping defects under nutritional stress conditions of glucose starvation or amino acid starvation. These findings provide evidence that 5'-end capping is not a constitutive process that necessarily always proceeds to completion and demonstrates that Rai1 has an essential role in clearing mRNAs with aberrant 5'-end caps. We propose that Rai1 is involved in an as yet uncharacterized quality control process that ensures mRNA 5'-end integrity by an aberrant-cap-mediated mRNA decay mechanism.

Links

PubMed PMC2948066 Online version:10.1038/nature09338

Keywords

5' Untranslated Regions/genetics; Amino Acids/deficiency; Amino Acids/metabolism; Endoribonucleases/metabolism; Exoribonucleases/metabolism; Glucose/deficiency; Glucose/metabolism; Guanosine/analogs & derivatives; Guanosine/metabolism; Hydrolysis; Methylation; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; RNA Caps/genetics; RNA Caps/metabolism; RNA Stability; RNA, Fungal/genetics; RNA, Fungal/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Stress, Physiological/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:DXO

involved_in

GO:0000956: nuclear-transcribed mRNA catabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:DXO

involved_in

GO:0031087: deadenylation-independent decapping of nuclear-transcribed mRNA

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:DXO

enables

GO:0034353: RNA pyrophosphohydrolase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:DXO

enables

GO:1990174: phosphodiesterase decapping endonuclease activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:DXO

enables

GO:1990174: phosphodiesterase decapping endonuclease activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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