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PMID:20739305

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Citation

Peters, S, Egert, A, Stieger, B and Keller, F (2010) Functional identification of Arabidopsis ATSIP2 (At3g57520) as an alkaline α-galactosidase with a substrate specificity for raffinose and an apparent sink-specific expression pattern. Plant Cell Physiol. 51:1815-9

Abstract

Arabidopsis ATSIP2 has recently been suggested to be a raffinose synthase gene. However, it has high amino acid identity to functionally characterized alkaline α-galactosidases from Cucumis melo and Zea mays. Using the Sf9 insect cell expression system, we demonstrate that recombinant ATSIP2 is a genuine alkaline α-galactosidase with a distinct substrate specificity for raffinose, and not a raffinose synthase. A β-glucuronidase reporter construct using the ATSIP2 promoter shows that ATSIP2 is strongly expressed in sink tissues of Arabidopsis, i.e. sink leaves and non-xylem parts of the root stele, suggesting a physiological function in raffinose phloem unloading.

Links

PubMed Online version:10.1093/pcp/pcq127

Keywords

Animals; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cell Line; Gene Expression Regulation, Plant; Insects/cytology; Phloem/metabolism; Promoter Regions, Genetic; RNA, Plant/genetics; Raffinose/metabolism; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Substrate Specificity; alpha-Galactosidase/genetics; alpha-Galactosidase/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:RFS2

NOT|enables

GO:0047274: galactinol-sucrose galactosyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:RFS2

enables

GO:0052692: raffinose alpha-galactosidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:RFS2

acts_upstream_of_or_within

GO:0034484: raffinose catabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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