PMID:20729808

Citation	Bernard, D, Prasanth, KV, Tripathi, V, Colasse, S, Nakamura, T, Xuan, Z, Zhang, MQ, Sedel, F, Jourdren, L, Coulpier, F, Triller, A, Spector, DL and Bessis, A (2010) A long nuclear-retained non-coding RNA regulates synaptogenesis by modulating gene expression. EMBO J. 29:3082-93
Abstract	A growing number of long nuclear-retained non-coding RNAs (ncRNAs) have recently been described. However, few functions have been elucidated for these ncRNAs. Here, we have characterized the function of one such ncRNA, identified as metastasis-associated lung adenocarcinoma transcript 1 (Malat1). Malat1 RNA is expressed in numerous tissues and is highly abundant in neurons. It is enriched in nuclear speckles only when RNA polymerase II-dependent transcription is active. Knock-down studies revealed that Malat1 modulates the recruitment of SR family pre-mRNA-splicing factors to the transcription site of a transgene array. DNA microarray analysis in Malat1-depleted neuroblastoma cells indicates that Malat1 controls the expression of genes involved not only in nuclear processes, but also in synapse function. In cultured hippocampal neurons, knock-down of Malat1 decreases synaptic density, whereas its over-expression results in a cell-autonomous increase in synaptic density. Our results suggest that Malat1 regulates synapse formation by modulating the expression of genes involved in synapse formation and/or maintenance.
Links	PubMed PMC2944070 Online version:10.1038/emboj.2010.199
Keywords	Animals; Biological Markers/metabolism; Blotting, Northern; Bone Neoplasms/genetics; Bone Neoplasms/metabolism; Bone Neoplasms/pathology; Cell Nucleus/genetics; Cell Nucleus/metabolism; Cells, Cultured; Gene Expression Profiling; Gene Expression Regulation/physiology; Hippocampus/cytology; Hippocampus/metabolism; Humans; Mice; Neuroblastoma/genetics; Neuroblastoma/metabolism; Neuroblastoma/pathology; Neurogenesis/physiology; Neurons/cytology; Neurons/metabolism; Oligonucleotide Array Sequence Analysis; Osteosarcoma/genetics; Osteosarcoma/metabolism; Osteosarcoma/pathology; RNA Precursors/genetics; RNA Splicing/genetics; RNA, Messenger/genetics; RNA, Nuclear/physiology; Reverse Transcriptase Polymerase Chain Reaction; Synapses/genetics; Transcription Factors/genetics; Transcription Factors/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:SRSF1	located_in	GO:0016607: nuclear speck	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete

References

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