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PMID:20728445
| Citation |
Koseki, T, Mihara, K, Murayama, T and Shiono, Y (2010) A novel Aspergillus oryzae esterase that hydrolyzes 4-hydroxybenzoic acid esters. FEBS Lett. 584:4032-6 |
|---|---|
| Abstract |
In this study we report the biochemical characterization of a hypothetical protein from Aspergillus oryzae exhibiting sequence identity with feruloyl esterase and tannase from the genus Aspergillus. The purified recombinant protein showed a hydrolytic activity toward the ethyl, propyl, or butyl esters of 4-hydroxybenzoic acid, but did not show feruloyl esterase or tannase activity. Finally, the enzyme decreased the antimicrobial activity of parabens against A. oryzae via hydrolysis of the ester bond present in butyl 4-hydroxybenzoic acid. |
| Links |
PubMed Online version:10.1016/j.febslet.2010.08.021 |
| Keywords |
Amino Acid Sequence; Antifungal Agents/chemistry; Antifungal Agents/metabolism; Antifungal Agents/pharmacology; Aspergillus oryzae/drug effects; Aspergillus oryzae/enzymology; Carboxylic Ester Hydrolases/classification; Carboxylic Ester Hydrolases/genetics; Carboxylic Ester Hydrolases/metabolism; Esters/chemistry; Esters/metabolism; Esters/pharmacology; Hydrolysis; Molecular Sequence Data; Parabens/chemistry; Parabens/metabolism; Parabens/pharmacology; Phylogeny; Recombinant Proteins/genetics; Recombinant Proteins/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0052689: carboxylic ester hydrolase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0052689: carboxylic ester hydrolase activity |
ECO:0000314: |
F |
Supplementary data 2. |
complete | ||||
See also
References
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