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PMID:20727857

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Citation

Motojima-Miyazaki, Y, Yoshida, M and Motojima, F (2010) Ribosomal protein L2 associates with E. coli HtpG and activates its ATPase activity. Biochem. Biophys. Res. Commun. 400:241-5

Abstract

Although eukaryotic Hsp90 has been studied extensively, the function of its bacterial homologue HtpG remains elusive. Here we report that 50S ribosomal protein L2 was found as an associated protein with His-tagged HtpG from Escherichia coli cultured in minimum medium at 45 °C. L2 specifically activated ATPase activity of HtpG, but other denatured proteins did not. The analysis using domain derivatives of HtpG and L2 showed that C-terminal domain of L2 and the middle to C-terminal domain of HtpG are important for interaction. At physiological salt concentration, L2 was denatured state and was recognized by HtpG as well as other chaperones, DnaK/DnaJ/GrpE and GroEL/GroES. The ATPase of HtpG at increasing concentration of L2 indicated that an L2 molecule bound to a dimer HtpG with apparent K(D) of 0.3 μM at 100mM KCl and 3.3 μM at 200 mM KCl.

Links

PubMed Online version:10.1016/j.bbrc.2010.08.047

Keywords

Adenosine Triphosphatases/metabolism; Adenosine Triphosphate/metabolism; Enzyme Activation; Escherichia coli/enzymology; Escherichia coli/growth & development; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; HSP90 Heat-Shock Proteins/genetics; HSP90 Heat-Shock Proteins/metabolism; Hydrolysis; Potassium Chloride/metabolism; Protein Structure, Tertiary; Ribosomal Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:HTPG

GO:0016887: ATPase activity

ECO:0000314:

F

Fig 2 shows ATPase activity in the presence of various proteins.

complete
CACAO 9616

ECOLI:HTPG

enables

GO:0016887: ATPase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

PSYA2:HTPG

GO:0005524: ATP binding

ECO:0000314:

F

Fig 2 shows ATPase activity in the presence of various proteins.

complete
CACAO 9571

See also

References

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