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PMID:20708585

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Citation

Blythe, SA, Cha, SW, Tadjuidje, E, Heasman, J and Klein, PS (2010) beta-Catenin primes organizer gene expression by recruiting a histone H3 arginine 8 methyltransferase, Prmt2. Dev. Cell 19:220-31

Abstract

An emerging concept in development is that transcriptional poising presets patterns of gene expression in a manner that reflects a cell's developmental potential. However, it is not known how certain loci are specified in the embryo to establish poised chromatin architecture as the developmental program unfolds. We find that, in the context of transcriptional quiescence prior to the midblastula transition in Xenopus, dorsal specification by the Wnt/beta-catenin pathway is temporally uncoupled from the onset of dorsal target gene expression, and that beta-catenin establishes poised chromatin architecture at target promoters. beta-catenin recruits the arginine methyltransferase Prmt2 to target promoters, thereby establishing asymmetrically dimethylated H3 arginine 8 (R8). Recruitment of Prmt2 to beta-catenin target genes is necessary and sufficient to establish the dorsal developmental program, indicating that Prmt2-mediated histone H3(R8) methylation plays a critical role downstream of beta-catenin in establishing poised chromatin architecture and marking key organizer genes for later expression.

Links

PubMed PMC2923644 Online version:10.1016/j.devcel.2010.07.007

Keywords

Animals; Arginine/metabolism; Body Patterning/physiology; Chromatin/chemistry; Chromatin/metabolism; Gene Expression Regulation, Developmental; Histones/genetics; Histones/metabolism; Molecular Sequence Data; Promoter Regions, Genetic; Protein-Arginine N-Methyltransferases/genetics; Protein-Arginine N-Methyltransferases/metabolism; RNA Polymerase II/metabolism; Wnt Proteins/genetics; Wnt Proteins/metabolism; Xenopus Proteins/genetics; Xenopus Proteins/metabolism; Xenopus laevis/anatomy & histology; Xenopus laevis/embryology; Xenopus laevis/physiology; beta Catenin/genetics; beta Catenin/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

XENLA:ANM2

involved_in

GO:0016571: histone methylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

XENLA:ANM2

enables

GO:0008469: histone-arginine N-methyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

XENLA:ANM2

involved_in

GO:0048588: developmental cell growth

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

XENLA:CTNB1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9R144

F

Seeded From UniProt

complete

MOUSE:ANM2

involved_in

GO:0016571: histone methylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:ANM2

enables

GO:0008469: histone-arginine N-methyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:ANM2

enables

GO:0008013: beta-catenin binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P26233

F

Seeded From UniProt

complete


See also

References

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