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PMID:20671110

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Citation

Jiang, L, Yuan, L, Xia, M and Makaroff, CA (2010) Proper levels of the Arabidopsis cohesion establishment factor CTF7 are essential for embryo and megagametophyte, but not endosperm, development. Plant Physiol. 154:820-32

Abstract

CTF7 is an essential gene in yeast that is required for the formation of sister chromatid cohesion. While recent studies have provided insights into how sister chromatid cohesion is established, less is known about how specifically CTF7 facilitates the formation of cohesion, and essentially nothing is known about how sister chromatid cohesion is established in plants. In this report, we describe the isolation and characterization of CTF7 from Arabidopsis (Arabidopsis thaliana). Arabidopsis CTF7 is similar to Saccharomyces cerevisiae CTF7 in that it lacks an amino-terminal extension, exhibits acetyltransferase activity, and can complement a yeast ctf7 temperature-sensitive mutation. CTF7 transcripts are found throughout the plant, with the highest levels present in buds. Seeds containing T-DNA insertions in CTF7 exhibit mitotic defects in the zygote. Interestingly, the endosperm developed normally in ctf7 seeds, suggesting that CTF7 is not essential for mitosis in endosperm nuclei. Minor defects were observed in female gametophytes of ctf7(+/-) plants, and plants that overexpress CTF7 exhibited female gametophyte lethality. Pollen development appeared normal in both CTF7 knockout and overexpression plants. Therefore, proper levels of CTF7 are critical for female gametophyte and embryo development but not for the establishment of mitotic cohesion during microgametogenesis or during endosperm development.

Links

PubMed PMC2949036 Online version:10.1104/pp.110.157560

Keywords

Acetyltransferases/genetics; Acetyltransferases/metabolism; Amino Acid Sequence; Arabidopsis/embryology; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; DNA, Bacterial/genetics; DNA, Plant/genetics; Endosperm/genetics; Endosperm/growth & development; Gene Knockout Techniques; Genes, Essential; Genetic Complementation Test; Molecular Sequence Data; Mutagenesis, Insertional; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Ovule/embryology; Ovule/genetics; Pollen/genetics; Pollen/growth & development; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:CTF7

enables

GO:0016407: acetyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:CTF7

acts_upstream_of_or_within

GO:0007062: sister chromatid cohesion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CTF7

acts_upstream_of_or_within

GO:0009793: embryo development ending in seed dormancy

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CTF7

acts_upstream_of_or_within

GO:0009553: embryo sac development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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