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PMID:20668183
| Citation |
Bozdagi, O, Wang, XB, Nikitczuk, JS, Anderson, TR, Bloss, EB, Radice, GL, Zhou, Q, Benson, DL and Huntley, GW (2010) Persistence of coordinated long-term potentiation and dendritic spine enlargement at mature hippocampal CA1 synapses requires N-cadherin. J. Neurosci. 30:9984-9 |
|---|---|
| Abstract |
Persistent changes in spine shape are coupled to long-lasting synaptic plasticity in hippocampus. The molecules that coordinate such persistent structural and functional plasticity are unknown. Here, we generated mice in which the cell adhesion molecule N-cadherin was conditionally ablated from postnatal, excitatory synapses in hippocampus. We applied to adult mice of either sex a combination of whole-cell recording, two-photon microscopy, and spine morphometric analysis to show that postnatal ablation of N-cadherin has profound effects on the stability of coordinated spine enlargement and long-term potentiation (LTP) at mature CA1 synapses, with no effects on baseline spine density or morphology, baseline properties of synaptic neurotransmission, or long-term depression. Thus, N-cadherin couples persistent spine structural modifications with long-lasting synaptic functional modifications associated selectively with LTP, revealing unexpectedly distinct roles at mature synapses in comparison with earlier, broader functions in synapse and spine development. |
| Links |
PubMed PMC2921177 Online version:10.1523/JNEUROSCI.1223-10.2010 |
| Keywords |
Action Potentials/physiology; Animals; Biophysics/methods; CA1 Region, Hippocampal/cytology; Cadherins/deficiency; Cadherins/metabolism; Dendritic Spines/physiology; Electric Stimulation/methods; Excitatory Postsynaptic Potentials/genetics; In Vitro Techniques; Long-Term Potentiation/physiology; Mice; Mice, Inbred C57BL; Mice, Knockout; Microscopy, Immunoelectron/methods; Patch-Clamp Techniques/methods; Pyramidal Cells/ultrastructure; Statistics, Nonparametric; Synapses/metabolism; Synapses/ultrastructure |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
part_of |
GO:0014069: postsynaptic density |
ECO:0000314: direct assay evidence used in manual assertion |
C |
part_of:(UBERON:0000955) |
Seeded From UniProt |
complete | ||
Notes
See also
References
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