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PMID:20667964

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Citation

Chae, K, Gonong, BJ, Kim, SC, Kieslich, CA, Morikis, D, Balasubramanian, S and Lord, EM (2010) A multifaceted study of stigma/style cysteine-rich adhesin (SCA)-like Arabidopsis lipid transfer proteins (LTPs) suggests diversified roles for these LTPs in plant growth and reproduction. J. Exp. Bot. 61:4277-90

Abstract

Lily stigma/style cysteine-rich adhesin (SCA), a plant lipid transfer protein (LTP) which is secreted into the extracellular matrix, functions in pollen tube guidance in fertilization. A gain-of-function mutant (ltp5-1) for Arabidopsis LTP5, an SCA-like molecule, was recently shown to display defects in sexual reproduction. In the current study, it is reported that ltp5-1 plants have dwarfed primary shoots, delayed hypocotyl elongation, various abnormal tissue fusions, and display multibranching. These mutant phenotypes in vegetative growth are recessive. No abnormality was found in ltp5-1/+ plants. In a phylogenetic analysis of plant LTPs, SCA-like Arabidopsis LTPs were classified with conventional plant LTPs. Homology modelling-based electrostatic similarity index (ESI) clustering was used to show diversity in spatial distributions of electrostatic potentials of SCA-like LTPs, suggestive of their various roles in interaction in the extracellular matrix space. β-Glucuronidase (GUS) analysis showed that SCA-like Arabidopsis LTP genes are diversely present in various tissues. LTP4 was found specifically in the guard cells and LTP6 in trichomes as well as in other tissues. LTP1 levels were specifically abundant in the stigma, and both LTP3 and LTP6 in the ovules. LTP2 and LTP4 gene levels were up-regulated in whole seedlings with 20% polyethylene glycol (PEG) and 300 mM NaCl treatments, respectively. LTP5 was up-regulated in the hypocotyl with 3 d dark growth conditions. LTP6 was specifically expressed in the tip of the cotyledon under drought stress conditions. The results suggest that SCA-like Arabidopsis LTPs are multifunctional, with diversified roles in plant growth and reproduction.

Links

PubMed PMC2955742 Online version:10.1093/jxb/erq228

Keywords

Antigens, Plant/chemistry; Antigens, Plant/classification; Antigens, Plant/genetics; Antigens, Plant/metabolism; Arabidopsis/cytology; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/metabolism; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/classification; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Carrier Proteins/chemistry; Carrier Proteins/classification; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cluster Analysis; Cysteine/metabolism; Flowers/cytology; Flowers/genetics; Flowers/metabolism; Gene Expression Regulation, Plant; Genes, Plant/genetics; Mutation/genetics; Phylogeny; Plant Proteins/chemistry; Plant Proteins/classification; Plant Proteins/genetics; Plant Proteins/metabolism; Reproduction/genetics; Seedling/growth & development; Seedling/metabolism; Stress, Physiological/genetics; Structural Homology, Protein

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:F4IXC6

acts_upstream_of_or_within

GO:0009414: response to water deprivation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NLTP4

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NLTP4

acts_upstream_of_or_within

GO:0009414: response to water deprivation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NLTP2

acts_upstream_of_or_within

GO:0009414: response to water deprivation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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