GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:20663920

From GONUTS
Jump to: navigation, search
Citation

Hofmann, ER, Nallar, SC, Lin, L, D'Cunha, J, Lindner, DJ, Weihua, X and Kalvakolanu, DV (2010) Identification and characterization of GRIM-1, a cell-death-associated gene product. J. Cell. Sci. 123:2781-91

Abstract

Using a genome-wide technical knockout, we isolated a newly identified set of GRIM (genes associated with retinoid-interferon-induced mortality) genes; GRIM genes mediate IFN- and retinoic-acid (RA)-induced cell death. Here, we describe the isolation and characterization of one such gene, GRIM-1. Three proteins, with identical C-termini, were produced from the GRIM-1 open reading frame when this gene was transcribed and translated in vitro. These protein isoforms, designated GRIM-1alpha, GRIM-1beta and GRIM-1gamma, differentially suppressed growth via apoptosis in various cell lines. We also show that a caspase-dependent mechanism generates the proapoptotic GRIM-1 isoforms. Lastly, GRIM-1 isoforms differentially blocked maturation of 18S ribosomal RNA, consistent with their respective growth-suppressive ability. Together, these studies identified a novel protein involved in growth suppression and cell death.

Links

PubMed PMC2915880 Online version:10.1242/jcs.070250

Keywords

Animals; Apoptosis/drug effects; Apoptosis/genetics; Apoptosis Regulatory Proteins/biosynthesis; Apoptosis Regulatory Proteins/genetics; Caspase 9/metabolism; Cell Death/drug effects; Cell Death/genetics; Cell Growth Processes/genetics; Gene Expression Regulation; Gene Knockout Techniques; HeLa Cells; Humans; Interferon-beta/pharmacology; Mice; Mice, Nude; Protein Isoforms; Proteins/genetics; RNA, Ribosomal/genetics; RNA, Ribosomal/metabolism; Tretinoin/pharmacology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:SHQ1

acts_upstream_of_or_within

GO:0043065: positive regulation of apoptotic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:SHQ1

acts_upstream_of_or_within

GO:2000233: negative regulation of rRNA processing

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CASP9

enables

GO:0008233: peptidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.