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PMID:20627947

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Citation

Satoh-Cruz, M, Crofts, AJ, Takemoto-Kuno, Y, Sugino, A, Washida, H, Crofts, N, Okita, TW, Ogawa, M, Satoh, H and Kumamaru, T (2010) Protein disulfide isomerase like 1-1 participates in the maturation of proglutelin within the endoplasmic reticulum in rice endosperm. Plant Cell Physiol. 51:1581-93

Abstract

The rice esp2 mutation was previously characterized by the abnormal accumulation of elevated levels of proglutelin and the absence of an endosperm-specific protein disulfide isomerase like (PDIL1-1). Here we show that Esp2 is the structural gene for PDIL1-1 and that this lumenal chaperone is asymmetrically distributed within the cortical endoplasmic reticulum (ER) and largely restricted to the cisternal ER. Temporal studies indicate that PDIL1-1 is essential for the maturation of proglutelin only when its rate of synthesis significantly exceeds its export from the ER, a condition resulting in its build up in the ER lumen and the induction of ER quality control processes which lower glutelin levels as well as those of the other storage proteins. As proglutelin is initially synthesized on the cisternal ER, its deposition within prolamine protein bodies in esp2 suggests that PDIL1-1 helps retain proglutelin in the cisternal ER lumen until it attains competence for ER export and, thereby, indirectly preventing heterotypic interactions with prolamine polypeptides.

Links

PubMed Online version:10.1093/pcp/pcq098

Keywords

Endoplasmic Reticulum/metabolism; Endosperm/metabolism; Gene Dosage; Glutens/metabolism; Oryza sativa/enzymology; Oryza sativa/genetics; Protein Disulfide-Isomerases/genetics; Protein Disulfide-Isomerases/metabolism; Seed Storage Proteins/genetics; Seed Storage Proteins/metabolism; Sequence Analysis, DNA

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ORYSJ:PDI11

located_in

GO:0005788: endoplasmic reticulum lumen

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ORYSJ:PDI11

part_of

GO:0005788: endoplasmic reticulum lumen

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ORYSJ:PDI11

involved_in

GO:0034975: protein folding in endoplasmic reticulum

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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