PMID:20622453

Citation	Kim, SW, Fushinobu, S, Zhou, S, Wakagi, T and Shoun, H (2010) The possible involvement of copper-containing nitrite reductase (NirK) and flavohemoglobin in denitrification by the fungus Cylindrocarpon tonkinense. Biosci. Biotechnol. Biochem. 74:1403-7
Abstract	The occurrence of denitrification and nitrate respiration among eukaryotes has been established during the last few decades. However, denitrification-related eukaryotic genes have been isolated from only a few fungi, and eukaryotic denitrification (or nitrate respiration) is still inadequately understood. In this study, we identified genes that were up-regulated under denitrifying conditions in the fungus Cylindrocarpon tonkinense using the suppression subtraction hybridization technique, and the expression patterns of these genes were characterized by Northern analysis. We identified copper-containing nitrite reductase, cytochrome P450 nitric oxide reductase, flavohemoglobin (Fhb), and formate/nitrite transporter homolog genes as possibly involved in fungal denitrification. Our results concerning the involvement of Fhb and formate/nitrite transporter perhaps provide new insight into the fungal denitrification system.
Links	PubMed
Keywords	Cloning, Molecular; Copper/metabolism; Fungal Proteins/metabolism; Fusarium/enzymology; Fusarium/genetics; Fusarium/metabolism; Gene Expression Regulation, Fungal; Nitrite Reductases/chemistry; Nitrite Reductases/genetics; Nitrite Reductases/metabolism; Sequence Analysis, DNA
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
FUSOX:A9XR52		GO:0019333: denitrification pathway	ECO:0000255:	PMID:9254694	P		Table 2.	complete

References

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