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PMID:20620953

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Citation

Prestel, M, Feller, C, Straub, T, Mitlöhner, H and Becker, PB (2010) The activation potential of MOF is constrained for dosage compensation. Mol. Cell 38:815-26

Abstract

The H4K16 acetyltransferase MOF plays a crucial role in dosage compensation in Drosophila but has additional, global functions. We compared the molecular context and effect of MOF in male and female flies, combining chromosome-wide mapping and transcriptome studies with analyses of defined reporter loci in transgenic flies. MOF distributes dynamically between two complexes, the dosage compensation complex and a complex containing MBD-R2, a global facilitator of transcription. These different targeting principles define the distribution of MOF between the X chromosome and autosomes and at transcription units with 5' or 3' enrichment. The male X chromosome differs from all other chromosomes in that H4K16 acetylation levels do not correlate with transcription output. The reconstitution of this phenomenon at a model locus revealed that the activation potential of MOF is constrained in male cells in the context of the DCC to arrive at the 2-fold activation of transcription characteristic of dosage compensation.

Links

PubMed Online version:10.1016/j.molcel.2010.05.022

Keywords

Acetylation; Animals; Dosage Compensation, Genetic; Drosophila/genetics; Drosophila/metabolism; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Female; Gene Expression Profiling; Histone Acetyltransferases/genetics; Histone Acetyltransferases/metabolism; Male; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Sex Factors; Transcriptional Activation; X Chromosome/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:MOF

part_of

GO:0005667: transcription regulator complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:MOF

part_of

GO:0016456: X chromosome located dosage compensation complex, transcription activating

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q7KSN0

located_in

GO:0035327: transcriptionally active chromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VGA4

located_in

GO:0035327: transcriptionally active chromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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