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PMID:20603001

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Citation

Jin, H, White, SR, Shida, T, Schulz, S, Aguiar, M, Gygi, SP, Bazan, JF and Nachury, MV (2010) The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia. Cell 141:1208-19

Abstract

The BBSome is a complex of Bardet-Biedl Syndrome (BBS) proteins that shares common structural elements with COPI, COPII, and clathrin coats. Here, we show that the BBSome constitutes a coat complex that sorts membrane proteins to primary cilia. The BBSome is the major effector of the Arf-like GTPase Arl6/BBS3, and the BBSome and GTP-bound Arl6 colocalize at ciliary punctae in an interdependent manner. Strikingly, Arl6(GTP)-mediated recruitment of the BBSome to synthetic liposomes produces distinct patches of polymerized coat apposed onto the lipid bilayer. Finally, the ciliary targeting signal of somatostatin receptor 3 needs to be directly recognized by the BBSome in order to mediate targeting of membrane proteins to cilia. Thus, we propose that trafficking of BBSome cargoes to cilia entails the coupling of BBSome coat polymerization to the recognition of sorting signals by the BBSome.

Links

PubMed PMC2898735 Online version:10.1016/j.cell.2010.05.015

Keywords

ADP-Ribosylation Factors/metabolism; Animals; Bardet-Biedl Syndrome/metabolism; Cattle; Cell Membrane/metabolism; Cilia/metabolism; Humans; Liposomes/metabolism; Mice; Multiprotein Complexes/metabolism; Phospholipids/metabolism; Protein Folding; Protein Transport; Receptors, Somatostatin/metabolism; Retina/metabolism; Tissue Extracts/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BOVIN:ARL6

located_in

GO:0005930: axoneme

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BOVIN:ARL6

enables

GO:0005543: phospholipid binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

BOVIN:ARL6

located_in

GO:0005879: axonemal microtubule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BOVIN:ARL6

involved_in

GO:0006612: protein targeting to membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

BOVIN:ARL6

part_of

GO:0030117: membrane coat

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BOVIN:ARL6

involved_in

GO:0051258: protein polymerization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

BOVIN:ARL6

enables

GO:0062063: BBSome binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:BBS1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9H0F7

F

Seeded From UniProt

complete

MOUSE:BBS5

part_of

GO:0034464: BBSome

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:17168)

Seeded From UniProt

complete

HUMAN:ARL6

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q8NFJ9

F

Seeded From UniProt

complete


See also

References

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