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PMID:20585622

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Citation

Schmidt, JM, Good, RT, Appleton, B, Sherrard, J, Raymant, GC, Bogwitz, MR, Martin, J, Daborn, PJ, Goddard, ME, Batterham, P and Robin, C (2010) Copy number variation and transposable elements feature in recent, ongoing adaptation at the Cyp6g1 locus. PLoS Genet. 6:e1000998

Abstract

The increased transcription of the Cyp6g1 gene of Drosophila melanogaster, and consequent resistance to insecticides such as DDT, is a widely cited example of adaptation mediated by cis-regulatory change. A fragment of an Accord transposable element inserted upstream of the Cyp6g1 gene is causally associated with resistance and has spread to high frequencies in populations around the world since the 1940s. Here we report the existence of a natural allelic series at this locus of D. melanogaster, involving copy number variation of Cyp6g1, and two additional transposable element insertions (a P and an HMS-Beagle). We provide evidence that this genetic variation underpins phenotypic variation, as the more derived the allele, the greater the level of DDT resistance. Tracking the spatial and temporal patterns of allele frequency changes indicates that the multiple steps of the allelic series are adaptive. Further, a DDT association study shows that the most resistant allele, Cyp6g1-[BP], is greatly enriched in the top 5% of the phenotypic distribution and accounts for approximately 16% of the underlying phenotypic variation in resistance to DDT. In contrast, copy number variation for another candidate resistance gene, Cyp12d1, is not associated with resistance. Thus the Cyp6g1 locus is a major contributor to DDT resistance in field populations, and evolution at this locus features multiple adaptive steps occurring in rapid succession.

Links

PubMed PMC2891717 Online version:10.1371/journal.pgen.1000998

Keywords

Adaptation, Biological; Alleles; Animals; Animals, Genetically Modified; Cytochrome P-450 Enzyme System/genetics; DNA Copy Number Variations; DNA Transposable Elements; Drosophila Proteins/genetics; Drosophila melanogaster/genetics; Genetic Loci; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:CP6G1

involved_in

GO:0046680: response to DDT

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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