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PMID:20577052

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Citation

Mariño, G, Fernández, AF, Cabrera, S, Lundberg, YW, Cabanillas, R, Rodríguez, F, Salvador-Montoliu, N, Vega, JA, Germanà, A, Fueyo, A, Freije, JM and López-Otín, C (2010) Autophagy is essential for mouse sense of balance. J. Clin. Invest. 120:2331-44

Abstract

Autophagy is an evolutionarily conserved process that is essential for cellular homeostasis and organismal viability in eukaryotes. However, the extent of its functions in higher-order processes of organismal physiology and behavior is still unknown. Here, we report that autophagy is essential for the maintenance of balance in mice and that its deficiency leads to severe balance disorders. We generated mice deficient in autophagin-1 protease (Atg4b) and showed that they had substantial systemic reduction of autophagic activity. Autophagy reduction occurred through defective proteolytic processing of the autophagosome component LC3 and its paralogs, which compromised the rate of autophagosome maturation. Despite their viability, Atg4b-null mice showed unusual patterns of behavior that are common features of inner ear pathologies. Consistent with this, Atg4b-null mice showed defects in the development of otoconia, organic calcium carbonate crystals essential for sense of balance (equilibrioception). Furthermore, these abnormalities were exacerbated in Atg5-/- mice, which completely lack the ability to perform autophagy, confirming that autophagic activity is necessary for otoconial biogenesis. Autophagy deficiency also led to impaired secretion and assembly of otoconial core proteins, thus hampering otoconial development. Taken together, these results describe an essential role for autophagy in inner ear development and equilibrioception and open new possibilities for understanding and treating human balance disorders, which are of growing relevance among the elderly population.

Links

PubMed PMC2898610 Online version:10.1172/JCI42601

Keywords

Animals; Autophagy/physiology; Eukaryota; Lysosomes/metabolism; Mice; Mice, Knockout; Mice, Transgenic; Otolithic Membrane/metabolism; Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:ATG5

acts_upstream_of_or_within

GO:0048840: otolith development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3612279

P

Seeded From UniProt

complete

MOUSE:ATG7

acts_upstream_of_or_within

GO:0021680: cerebellar Purkinje cell layer development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3587769

P

Seeded From UniProt

complete

MOUSE:ATG7

acts_upstream_of_or_within

GO:0006914: autophagy

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3587769

P

Seeded From UniProt

complete

MOUSE:ATG7

acts_upstream_of_or_within

GO:0021955: central nervous system neuron axonogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3587769

P

Seeded From UniProt

complete


See also

References

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