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PMID:20549745

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Citation

Flores, MV, Hall, CJ, Crosier, KE and Crosier, PS (2010) Visualization of embryonic lymphangiogenesis advances the use of the zebrafish model for research in cancer and lymphatic pathologies. Dev. Dyn. 239:2128-35

Abstract

Lymphangiogenesis induced during tumor growth contributes to metastasis. Genetic and chemical screens using the zebrafish model have the potential to enhance our understanding of lymphangiogenesis, and lead to the discovery of pharmacological agents with activity in the lymphatic system. Large-scale screening of lymphatic development in the whole zebrafish embryo requires a specific lymphatic endothelial cell marker. We isolated the zebrafish ortholog of Lyve1, and analyzed its expression pattern during embryogenesis, and under conditions where key regulators of lymphangiogenesis such as Prox1 and VegfC were depleted. Like humans, zebrafish embryos form lymph sacs, lymphangioblasts arise from venous endothelia, and they form asymmetric left and right collecting ducts. By monitoring the earliest lymphatic sprouting in the head, a pilot drug assay was performed showing rapamycin, an inhibitor of mammalian lymphangiogenesis, can also suppress zebrafish lymphangiogenesis. This work opens up a novel opportunity to further the understanding of, and potentially manipulate, human lymphangiogenesis.

Links

PubMed Online version:10.1002/dvdy.22328

Keywords

Animals; In Situ Hybridization; Lymphangiogenesis/drug effects; Lymphangiogenesis/genetics; Lymphangiogenesis/physiology; Mice; Neoplasms/pathology; Sirolimus/pharmacology; Zebrafish/embryology; Zebrafish/metabolism; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:F1QAE1

involved_in

GO:0001946: lymphangiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050225-3

P

Seeded From UniProt

complete

DANRE:F1QXI5

involved_in

GO:0007368: determination of left/right symmetry

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-080722-6

P

Seeded From UniProt

complete

DANRE:F6P2I4

involved_in

GO:0001946: lymphangiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100707-6

P

Seeded From UniProt

complete

DANRE:F6P2I4

involved_in

GO:0001946: lymphangiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100707-5

P

Seeded From UniProt

complete

DANRE:Q0IIR3

involved_in

GO:0007368: determination of left/right symmetry

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-080722-6

P

Seeded From UniProt

complete

DANRE:Q288P3

involved_in

GO:0007368: determination of left/right symmetry

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-080722-6

P

Seeded From UniProt

complete

DANRE:Q502S0

involved_in

GO:0001946: lymphangiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050225-3

P

Seeded From UniProt

complete

DANRE:Q7T3I6

involved_in

GO:0001946: lymphangiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100707-6

P

Seeded From UniProt

complete

DANRE:Q7T3I6

involved_in

GO:0001946: lymphangiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100707-5

P

Seeded From UniProt

complete

DANRE:Q90ZL7

involved_in

GO:0001946: lymphangiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050225-3

P

Seeded From UniProt

complete

DANRE:Q9YHZ4

involved_in

GO:0001946: lymphangiogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050225-3

P

Seeded From UniProt

complete

DANRE:X2CSN0

involved_in

GO:0007368: determination of left/right symmetry

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-080722-6

P

Seeded From UniProt

complete


See also

References

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