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PMID:20547130

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Citation

Banovic, D, Khorramshahi, O, Owald, D, Wichmann, C, Riedt, T, Fouquet, W, Tian, R, Sigrist, SJ and Aberle, H (2010) Drosophila neuroligin 1 promotes growth and postsynaptic differentiation at glutamatergic neuromuscular junctions. Neuron 66:724-38

Abstract

Precise apposition of presynaptic and postsynaptic domains is a fundamental property of all neuronal circuits. Experiments in vitro suggest that Neuroligins and Neurexins function as key regulatory proteins in this process. In a genetic screen, we recovered several mutant alleles of Drosophila neuroligin 1 (dnlg1) that cause a severe reduction in bouton numbers at neuromuscular junctions (NMJs). In accord with reduced synapse numbers, these NMJs show reduced synaptic transmission. Moreover, lack of postsynaptic DNlg1 leads to deficits in the accumulation of postsynaptic glutamate receptors, scaffold proteins, and subsynaptic membranes, while increased DNlg1 triggers ectopic postsynaptic differentiation via its cytoplasmic domain. DNlg1 forms discrete clusters adjacent to postsynaptic densities. Formation of these clusters depends on presynaptic Drosophila Neurexin (DNrx). However, DNrx binding is not an absolute requirement for DNlg1 function. Instead, other signaling components are likely involved in DNlg1 transsynaptic functions, with essential interactions organized by the DNlg1 extracellular domain but also by the cytoplasmic domain.

Links

PubMed Online version:10.1016/j.neuron.2010.05.020

Keywords

Alleles; Animals; Cell Adhesion Molecules, Neuronal/genetics; Cell Adhesion Molecules, Neuronal/physiology; Cell Differentiation/genetics; Cell Differentiation/physiology; Drosophila; Drosophila Proteins/genetics; Drosophila Proteins/physiology; Mutation; Neuromuscular Junction/genetics; Neuromuscular Junction/physiology; Neuromuscular Junction/ultrastructure; Presynaptic Terminals/physiology; Presynaptic Terminals/ultrastructure; Receptors, Glutamate/genetics; Receptors, Glutamate/physiology; Receptors, Glutamate/ultrastructure; Synapses/genetics; Synapses/physiology; Synapses/ultrastructure; Synaptic Transmission/genetics; Synaptic Transmission/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:Q9VIC7

located_in

GO:0031594: neuromuscular junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VIC7

involved_in

GO:0007528: neuromuscular junction development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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