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PMID:20521084

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Citation

Wang, L, Ouyang, M, Li, Q, Zou, M, Guo, J, Ma, J, Lu, C and Zhang, L (2010) The Arabidopsis chloroplast ribosome recycling factor is essential for embryogenesis and chloroplast biogenesis. Plant Mol. Biol. 74:47-59

Abstract

To gain insight into the functions of the nuclear-encoded factors involved in chloroplast development, we characterized the high chlorophyll fluorescence and pale green mutant 108-1 (designated as hfp108-1) of Arabidopsis thaliana. Map-based cloning revealed that the mutant contains a tandem repeat of part of the sequence (including 116 nucleotides from 631 to 746 bp downstream of the ATG) of At3g63190, which encodes a chloroplast ribosome recycling factor homologue and was named AtcpRRF. The chloroplasts of hfp108-1 plants contain few internal thylakoid membranes and are severely defective in the accumulation of chloroplast-encoded proteins. In vivo labeling experiments showed a drastic decrease in the synthesis of the chloroplast-encoded proteins, which may be attributed primarily to reduced translation of the corresponding mRNA molecules. The level of the HFP108 transcript was greatly reduced in hfp108-1, so hfp108-1 showed a weak phenotype, and null alleles of HFP108 (hfp108-2) were embryonic lethal. Observations with cleared seeds in the same silique showed that homozygous hfp108-2 seeds were blocked at the heart stage and did not develop further. Thus, these results suggest that AtcpRRF is essential for embryogenesis and chloroplast biogenesis.

Links

PubMed Online version:10.1007/s11103-010-9653-0

Keywords

Amino Acid Sequence; Arabidopsis/embryology; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Base Sequence; Chloroplasts/genetics; Chloroplasts/metabolism; Chloroplasts/ultrastructure; Cloning, Molecular; DNA, Plant/genetics; Genes, Plant; Microscopy, Electron, Transmission; Molecular Sequence Data; Mutation; Plants, Genetically Modified; Ribosomal Proteins/genetics; Ribosomal Proteins/metabolism; Ribosomes/genetics; Ribosomes/metabolism; Sequence Homology, Amino Acid; Tandem Repeat Sequences

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:RRFC

involved_in

GO:0032544: plastid translation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RRFC

located_in

GO:0009507: chloroplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:RRFC

involved_in

GO:0009793: embryo development ending in seed dormancy

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RRFC

involved_in

GO:0009658: chloroplast organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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