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PMID:20516338

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Citation

Falcone Ferreyra, ML, Pezza, A, Biarc, J, Burlingame, AL and Casati, P (2010) Plant L10 ribosomal proteins have different roles during development and translation under ultraviolet-B stress. Plant Physiol. 153:1878-94

Abstract

Ribosomal protein L10 (RPL10) proteins are ubiquitous in the plant kingdom. Arabidopsis (Arabidopsis thaliana) has three RPL10 genes encoding RPL10A to RPL10C proteins, while two genes are present in the maize (Zea mays) genome (rpl10-1 and rpl10-2). Maize and Arabidopsis RPL10s are tissue-specific and developmentally regulated, showing high levels of expression in tissues with active cell division. Coimmunoprecipitation experiments indicate that RPL10s in Arabidopsis associate with translation proteins, demonstrating that it is a component of the 80S ribosome. Previously, ultraviolet-B (UV-B) exposure was shown to increase the expression of a number of maize ribosomal protein genes, including rpl10. In this work, we demonstrate that maize rpl10 genes are induced by UV-B while Arabidopsis RPL10s are differentially regulated by this radiation: RPL10A is not UV-B regulated, RPL10B is down-regulated, while RPL10C is up-regulated by UV-B in all organs studied. Characterization of Arabidopsis T-DNA insertional mutants indicates that RPL10 genes are not functionally equivalent. rpl10A and rpl10B mutant plants show different phenotypes: knockout rpl10A mutants are lethal, rpl10A heterozygous plants are deficient in translation under UV-B conditions, and knockdown homozygous rpl10B mutants show abnormal growth. Based on the results described here, RPL10 genes are not redundant and participate in development and translation under UV-B stress.

Links

PubMed PMC2923885 Online version:10.1104/pp.110.157057

Keywords

Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis/radiation effects; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Gene Expression Regulation, Developmental/radiation effects; Gene Expression Regulation, Plant/radiation effects; Gene Knockdown Techniques; Multigene Family; Mutagenesis, Insertional; Protein Biosynthesis; RNA, Plant/genetics; Ribosomal Proteins/genetics; Ribosomal Proteins/metabolism; Ultraviolet Rays; Zea mays/genetics; Zea mays/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:RL102

involved_in

GO:0010224: response to UV-B

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RL102

located_in

GO:0005840: ribosome

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:At3g25520

C

Seeded From UniProt

complete

ARATH:RL102

involved_in

GO:0032502: developmental process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RL101

located_in

GO:0005840: ribosome

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:At3g25520

C

Seeded From UniProt

complete

ARATH:RL101

involved_in

GO:0071493: cellular response to UV-B

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RL103

involved_in

GO:0010224: response to UV-B

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RL103

located_in

GO:0005840: ribosome

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:At3g25520

C

Seeded From UniProt

complete


See also

References

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