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PMID:20498018

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Citation

Chadrin, A, Hess, B, San Roman, M, Gatti, X, Lombard, B, Loew, D, Barral, Y, Palancade, B and Doye, V (2010) Pom33, a novel transmembrane nucleoporin required for proper nuclear pore complex distribution. J. Cell Biol. 189:795-811

Abstract

The biogenesis of nuclear pore complexes (NPCs) represents a paradigm for the assembly of high-complexity macromolecular structures. So far, only three integral pore membrane proteins are known to function redundantly in NPC anchoring within the nuclear envelope. Here, we describe the identification and functional characterization of Pom33, a novel transmembrane protein dynamically associated with budding yeast NPCs. Pom33 becomes critical for yeast viability in the absence of a functional Nup84 complex or Ndc1 interaction network, which are two core NPC subcomplexes, and associates with the reticulon Rtn1. Moreover, POM33 loss of function impairs NPC distribution, a readout for a subset of genes required for pore biogenesis, including members of the Nup84 complex and RTN1. Consistently, we show that Pom33 is required for normal NPC density in the daughter nucleus and for proper NPC biogenesis and/or stability in the absence of Nup170. We hypothesize that, by modifying or stabilizing the nuclear envelope-NPC interface, Pom33 may contribute to proper distribution and/or efficient assembly of nuclear pores.

Links

PubMed PMC2878943 Online version:10.1083/jcb.200910043

Keywords

Active Transport, Cell Nucleus/genetics; Amino Acid Sequence; Cell Proliferation; Endoplasmic Reticulum/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Molecular Sequence Data; Nuclear Pore/genetics; Nuclear Pore/metabolism; Nuclear Pore/ultrastructure; Nuclear Pore Complex Proteins/genetics; Nuclear Pore Complex Proteins/metabolism; Nucleocytoplasmic Transport Proteins/genetics; Nucleocytoplasmic Transport Proteins/metabolism; Phylogeny; Protein Binding/physiology; RNA, Messenger/metabolism; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Repressor Proteins/genetics; Repressor Proteins/metabolism; Ribonuclease III/genetics; Ribonuclease III/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Sequence Homology, Amino Acid; Telophase/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:NDC1

located_in

GO:0005635: nuclear envelope

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:PER33

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:PER33

located_in

GO:0005635: nuclear envelope

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:POM33

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:POM33

involved_in

GO:0006999: nuclear pore organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:POM33

located_in

GO:0031309: integral component of nuclear outer membrane

ECO:0000255: match to sequence model evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:POM33

located_in

GO:0005635: nuclear envelope

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:POM33

involved_in

GO:0051664: nuclear pore localization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:NU170

involved_in

GO:0051292: nuclear pore complex assembly

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000003946

P

Seeded From UniProt

complete

YEAST:NU170

enables

GO:0017056: structural constituent of nuclear pore

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000003946

F

Seeded From UniProt

complete


See also

References

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