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PMID:20484005

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Citation

dit Frey, NF, Muller, P, Jammes, F, Kizis, D, Leung, J, Perrot-Rechenmann, C and Bianchi, MW (2010) The RNA binding protein Tudor-SN is essential for stress tolerance and stabilizes levels of stress-responsive mRNAs encoding secreted proteins in Arabidopsis. Plant Cell 22:1575-91

Abstract

Tudor-SN (TSN) copurifies with the RNA-induced silencing complex in animal cells where, among other functions, it is thought to act on mRNA stability via the degradation of specific dsRNA templates. In plants, TSN has been identified biochemically as a cytoskeleton-associated RNA binding activity. In eukaryotes, it has recently been identified as a conserved primary target of programmed cell death-associated proteolysis. We have investigated the physiological role of TSN by isolating null mutations for two homologous genes in Arabidopsis thaliana. The double mutant tsn1 tsn2 displays only mild growth phenotypes under nonstress conditions, but germination, growth, and survival are severely affected under high salinity stress. Either TSN1 or TSN2 alone can complement the double mutant, indicating their functional redundancy. TSN accumulates heterogeneously in the cytosol and relocates transiently to a diffuse pattern in response to salt stress. Unexpectedly, stress-regulated mRNAs encoding secreted proteins are significantly enriched among the transcripts that are underrepresented in tsn1 tsn2. Our data also reveal that TSN is important for RNA stability of its targets. These findings show that TSN is essential for stress tolerance in plants and implicate TSN in new, potentially conserved mechanisms acting on mRNAs entering the secretory pathway.

Links

PubMed PMC2899877 Online version:10.1105/tpc.109.070680

Keywords

Adaptation, Physiological/drug effects; Adaptation, Physiological/genetics; Arabidopsis/cytology; Arabidopsis/drug effects; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis Proteins/genetics; Arabidopsis Proteins/secretion; Cytoplasm/drug effects; Cytoplasm/metabolism; Gene Expression Profiling; Gene Expression Regulation, Plant/drug effects; Genes, Plant/genetics; Genetic Complementation Test; Molecular Sequence Data; Mutation/genetics; Plant Roots/cytology; Plant Roots/drug effects; Plant Roots/genetics; Plant Roots/growth & development; Protein Transport/drug effects; RNA Stability/drug effects; RNA Stability/genetics; RNA, Messenger/genetics; RNA, Messenger/metabolism; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; RNA-Induced Silencing Complex/metabolism; Seeds/drug effects; Seeds/genetics; Sodium Chloride/pharmacology; Soil; Stress, Physiological/drug effects; Stress, Physiological/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:TSN1

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:TSN1

located_in

GO:0005829: cytosol

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:TSN1

involved_in

GO:0009651: response to salt stress

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:TSN1

located_in

GO:0005635: nuclear envelope

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:TSN1

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT5G61780

P

Seeded From UniProt

complete

ARATH:TSN1

enables

GO:0003723: RNA binding

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

ARATH:TSN2

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:TSN2

located_in

GO:0005829: cytosol

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:TSN2

located_in

GO:0005635: nuclear envelope

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:TSN2

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT5G07350

P

Seeded From UniProt

complete

ARATH:TSN2

enables

GO:0003723: RNA binding

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete


See also

References

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