GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:20479230

From GONUTS
Jump to: navigation, search
Citation

Assunção, AG, Herrero, E, Lin, YF, Huettel, B, Talukdar, S, Smaczniak, C, Immink, RG, van Eldik, M, Fiers, M, Schat, H and Aarts, MG (2010) Arabidopsis thaliana transcription factors bZIP19 and bZIP23 regulate the adaptation to zinc deficiency. Proc. Natl. Acad. Sci. U.S.A. 107:10296-301

Abstract

Zinc is an essential micronutrient for all living organisms. When facing a shortage in zinc supply, plants adapt by enhancing the zinc uptake capacity. The molecular regulators controlling this adaptation are not known. We present the identification of two closely related members of the Arabidopsis thaliana basic-region leucine-zipper (bZIP) transcription factor gene family, bZIP19 and bZIP23, that regulate the adaptation to low zinc supply. They were identified, in a yeast-one-hybrid screening, to associate to promoter regions of the zinc deficiency-induced ZIP4 gene of the Zrt- and Irt-related protein (ZIP) family of metal transporters. Although mutation of only one of the bZIP genes hardly affects plants, we show that the bzip19 bzip23 double mutant is hypersensitive to zinc deficiency. Unlike the wild type, the bzip19 bzip23 mutant is unable to induce the expression of a small set of genes that constitutes the primary response to zinc deficiency, comprising additional ZIP metal transporter genes. This set of target genes is characterized by the presence of one or more copies of a 10-bp imperfect palindrome in their promoter region, to which both bZIP proteins can bind. The bZIP19 and bZIP23 transcription factors, their target genes, and the characteristic cis zinc deficiency response elements they can bind to are conserved in higher plants. These findings are a significant step forward to unravel the molecular mechanism of zinc homeostasis in plants, allowing the improvement of zinc bio-fortification to alleviate human nutrition problems and phytoremediation strategies to clean contaminated soils.

Links

PubMed PMC2890486 Online version:10.1073/pnas.1004788107

Keywords

Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Base Sequence; Basic-Leucine Zipper Transcription Factors/genetics; Basic-Leucine Zipper Transcription Factors/metabolism; Conserved Sequence; DNA, Plant/genetics; Genes, Plant; Genetic Complementation Test; Humans; Mutagenesis, Insertional; Mutation; Phenotype; Plants/genetics; Plants, Genetically Modified; Promoter Regions, Genetic; Two-Hybrid System Techniques; Zinc/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:BZP23

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:AT1G10970

F

Seeded From UniProt

complete

ARATH:BZP23

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BZP23

enables

GO:0003677: DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:BZP19

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:AT1G10970

F

Seeded From UniProt

complete

ARATH:BZP19

acts_upstream_of_or_within

GO:0010043: response to zinc ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:BZP19

enables

GO:0003677: DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.