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PMID:20466844

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Citation

Wei, PC, Tan, F, Gao, XQ, Zhang, XQ, Wang, GQ, Xu, H, Li, LJ, Chen, J and Wang, XC (2010) Overexpression of AtDOF4.7, an Arabidopsis DOF family transcription factor, induces floral organ abscission deficiency in Arabidopsis. Plant Physiol. 153:1031-45

Abstract

After flower pollination, a programmed process called abscission occurs in which unwanted floral organs are actively shed from the main plant body. We found that a member of the DOF (for DNA binding with one finger) transcription factor family, Arabidopsis (Arabidopsis thaliana) DOF4.7, was expressed robustly in the abscission zone. The Arabidopsis 35S::AtDOF4.7 lines with constitutive expression of AtDOF4.7 exhibited an ethylene-independent floral organ abscission deficiency. In these lines, anatomical analyses showed that the formation of the abscission zone was normal. However, dissolution of the middle lamella failed to separate between the cell walls. AtDOF4.7 was identified as a nucleus-localized transcription factor. This protein had both in vitro and in vivo binding activity to typical DOF cis-elements in the promoter of an abscission-related polygalacturonase (PG) gene, PGAZAT. Overexpression of AtDOF4.7 resulted in down-regulation of PGAZAT. AtDOF4.7 interacted with another abscission-related transcription factor, Arabidopsis ZINC FINGER PROTEIN2. Taken together, our results suggest that AtDOF4.7 participates in the control of abscission as part of the transcription complex that directly regulates the expression of cell wall hydrolysis enzymes.

Links

PubMed PMC2899910 Online version:10.1104/pp.110.153247

Keywords

Arabidopsis/cytology; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/ultrastructure; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cell Nucleus/drug effects; Cell Nucleus/metabolism; Ethylenes/pharmacology; Flowers/cytology; Flowers/genetics; Flowers/growth & development; Flowers/ultrastructure; Gene Expression Regulation, Plant/drug effects; Organ Specificity/drug effects; Organ Specificity/genetics; Phenotype; Plants, Genetically Modified; Protein Binding/drug effects; Protein Transport/drug effects; Subcellular Fractions/drug effects; Subcellular Fractions/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Yeasts/drug effects; Yeasts/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:ZFP2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q84K52

F

Seeded From UniProt

complete

ARATH:ZFP2

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:DOF47

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q39261

F

Seeded From UniProt

complete

ARATH:DOF47

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:DOF47

acts_upstream_of_or_within

GO:0010227: floral organ abscission

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DOF47

enables

GO:0003677: DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:DOF47

acts_upstream_of_or_within

GO:0045893: positive regulation of transcription, DNA-templated

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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