GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:2045099

From GONUTS
Jump to: navigation, search
Citation

Oliva, D, Calì, L, Feo, S and Giallongo, A (1991) Complete structure of the human gene encoding neuron-specific enolase. Genomics 10:157-65

Abstract

At least three genes encode the different isoforms of the glycolytic enzyme enolase. We have isolated the gene for the human gamma- or neuron-specific enolase and determined the nucleotide sequence from upstream to the 5' end to beyond the polyadenylation site. The gene contains 12 exons distributed over 9213 nucleotides. Introns occur at positions identical to those reported for the homologous rat gene, as well as for the human alpha- or nonneuronal enolase gene, supporting the existence of a single ancestor for the members of this gene family. Primer extension analysis indicates that the gene has multiple start sites. The putative promoter region lacks canonical TATA and CAAT boxes, is very G + C-rich, and contains several potential regulatory sequences. Furthermore, an inverted Alu sequence is present approximately 572 nucleotides upstream of the major start site. A comparison of the 5'-flanking region of the human gamma-enolase gene with the same region of the rat gene revealed a high degree of sequence conservation.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Base Sequence; Blotting, Southern; DNA; Humans; Introns; Molecular Sequence Data; Multigene Family; Phosphopyruvate Hydratase/genetics; Promoter Regions, Genetic; Rats; Restriction Mapping; Sequence Homology, Nucleic Acid; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:ENOG

enables

GO:0004634: phosphopyruvate hydratase activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.