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PMID:20434988

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Citation

Morais-de-Sá, E, Mirouse, V and St Johnston, D (2010) aPKC phosphorylation of Bazooka defines the apical/lateral border in Drosophila epithelial cells. Cell 141:509-23

Abstract

Bazooka (PAR-3), PAR-6, and aPKC form a complex that plays a key role in the polarization of many cell types. In epithelial cells, however, Bazooka localizes below PAR-6 and aPKC at the apical/lateral junction. Here, we show that Baz is excluded from the apical aPKC domain in epithelia by aPKC phosphorylation, which disrupts the Baz/aPKC interaction. Removal of Baz from the complex is epithelial-specific because it also requires the Crumbs complex, which prevents the Baz/PAR-6 interaction. In the absence of Crumbs or aPKC phosphorylation of Baz, mislocalized Baz recruits adherens junction components apically, leading to a loss of the apical domain and an expansion of lateral. Thus, apical exclusion of Baz by Crumbs and aPKC defines the apical/lateral border. Although Baz acts as an aPKC targeting and specificity factor in nonepithelial cells, our results reveal that it performs a complementary function in positioning the adherens junction in epithelia.

Links

PubMed PMC2885938 Online version:10.1016/j.cell.2010.02.040

Keywords

Animals; Animals, Genetically Modified; Drosophila Proteins/metabolism; Drosophila melanogaster/cytology; Drosophila melanogaster/metabolism; Embryo, Nonmammalian/metabolism; Epithelial Cells/metabolism; Female; Intracellular Signaling Peptides and Proteins/metabolism; Ovarian Follicle/metabolism; Ovary/cytology; Ovary/metabolism; Phosphorylation; Protein Kinase C/metabolism; Protein Structure, Tertiary

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:APKC

enables

GO:0004674: protein serine/threonine kinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:APKC

located_in

GO:0045179: apical cortex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:APKC

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9VX75

F

Seeded From UniProt

complete

DROME:O96782

located_in

GO:0005912: adherens junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:O97111

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9VX75

F

Seeded From UniProt

complete

DROME:Q0KHR3

located_in

GO:0005912: adherens junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q86NX9

located_in

GO:0005912: adherens junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VX75

located_in

GO:0005912: adherens junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VX75

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:A1Z9X0

F

Seeded From UniProt

complete

DROME:Q9VX75

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O97111

F

Seeded From UniProt

complete

DROME:X2JCB3

located_in

GO:0005912: adherens junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:X2JFU8

located_in

GO:0005912: adherens junction

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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