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PMID:20420852

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Citation

Zhang, Z and Zhu, S (2010) Functional role of charged residues in drosomycin, a Drosophila antifungal peptide. Dev. Comp. Immunol. 34:953-8

Abstract

The functional importance of positively charged residues in antibacterial peptides (ABPs) has been well documented. However, their role in antifungal peptides (AFPs) has not been extensively evaluated. To address this question, we investigated the functional role of charged residues in drosomycin, a specific Drosophila antifungal peptide, using site-directed mutagenesis in combination with circular dichroism (CD) analysis and antifungal assays. As a result, we firstly identified five cationic residues (R6, K8, R20, R21 and K38) whose alterations significantly affected the antifungal activity. Intriguingly, two negatively charged residues (D1 and E25) are also recognized as functional determinants of drosomycin. This indicates that it is the location of these charged residues rather than net charges that is crucial for activity. These functional sites are located in different secondary structure elements, including the N-loop, alpha-helix and gamma-core regions, all highly exposed on the molecular surface, suggesting that drosomycin may bind to fungal targets through electrostatic interactions. Our work has implications for further modification of drosomycin to obtain new antifungal peptides with enhanced activity.

Links

PubMed Online version:10.1016/j.dci.2010.04.003

Keywords

Amino Acids, Basic/chemistry; Amino Acids, Basic/genetics; Amino Acids, Basic/metabolism; Animals; Antifungal Agents/chemistry; Antifungal Agents/immunology; Antifungal Agents/metabolism; Circular Dichroism; Drosophila; Drosophila Proteins/chemistry; Drosophila Proteins/genetics; Drosophila Proteins/immunology; Drosophila Proteins/metabolism; Fungi/growth & development; Fungi/immunology; Fungi/pathogenicity; Growth Inhibitors/chemistry; Growth Inhibitors/immunology; Growth Inhibitors/metabolism; Immunity/genetics; Mutagenesis, Site-Directed; Mutation/genetics; Mycoses/genetics; Mycoses/immunology; Mycoses/metabolism; Peptide Fragments/chemistry; Peptide Fragments/immunology; Peptide Fragments/metabolism; Protein Binding/genetics; Protein Binding/immunology; Protein Conformation

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:DMYC

involved_in

GO:0050832: defense response to fungus

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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