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PMID:20404084

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Citation

Kaufmann, I, White, E, Azad, A, Marguerat, S, Bähler, J and Proudfoot, NJ (2010) Transcriptional activation of the general amino acid permease gene per1 by the histone deacetylase Clr6 Is regulated by Oca2 kinase. Mol. Cell. Biol. 30:3396-410

Abstract

Expression of nitrogen metabolism genes is regulated by the quality of the nitrogen supply. Here, we describe a mechanism for the transcriptional regulation of the general amino acid permease gene per1 in Schizosaccharomyces pombe. We show that when ammonia is used as the nitrogen source, low levels of per1 are transcribed and histones in the coding and surrounding regions of per1 are acetylated. In the presence of proline, per1 transcription is upregulated and initiates from a more upstream site, generating 5'-extended mRNAs. Concomitantly, histones at per1 are deacetylated in a Clr6-dependent manner, suggesting a positive role for Clr6 in transcriptional regulation of per1. Upstream initiation and histone deactylation of per1 are constitutive in cells lacking the serine/threonine kinase oca2, indicating that Oca2 is a repressor of per1. Oca2 interacts with a protein homologous to the Saccharomyces cerevisiae transcriptional activator Cha4 and with Ago1. Loss of Cha4 or Ago1 causes aberrant induction of per1 under noninducing conditions, suggesting that these proteins are also involved in per1 regulation and hence in nitrogen utilization.

Links

PubMed PMC2897586 Online version:10.1128/MCB.00971-09

Keywords

Amino Acid Transport Systems/genetics; Amino Acid Transport Systems/metabolism; Ammonia/metabolism; Argonaute Proteins; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Gene Expression Regulation, Fungal; Genome, Fungal; Molecular Sequence Data; Nitrogen/metabolism; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism; RNA Interference; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Saccharomyces cerevisiae Proteins; Schizosaccharomyces/enzymology; Schizosaccharomyces/genetics; Schizosaccharomyces pombe Proteins/genetics; Schizosaccharomyces pombe Proteins/metabolism; Trans-Activators/genetics; Trans-Activators/metabolism; Transcriptional Activation

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

SCHPO:OCA2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PomBase:SPCC736.11

F

Seeded From UniProt

complete

SCHPO:OCA2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PomBase:SPBC1683.13c

F

Seeded From UniProt

complete

SCHPO:OCA2

involved_in

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • has_input:(PomBase:SPAP7G5.06)
  • part_of:(GO:0034763)

Seeded From UniProt

complete

SCHPO:OCA2

involved_in

GO:0006808: regulation of nitrogen utilization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:OCA2

enables

GO:0004674: protein serine/threonine kinase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:AGO1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PomBase:SPCC1020.10

F

Seeded From UniProt

complete

YEAST:HRK1

enables

GO:0004674: protein serine/threonine kinase activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:O59763

F

Seeded From UniProt

complete

SCHPO:PPK8

enables

GO:0004674: protein serine/threonine kinase activity

ECO:0000250: sequence similarity evidence used in manual assertion

PomBase:SPCC1020.10

F

Seeded From UniProt

complete

YEAST:RTK1

enables

GO:0004674: protein serine/threonine kinase activity

ECO:0000250: sequence similarity evidence used in manual assertion

UniProtKB:O59763

F

Seeded From UniProt

complete

SCHPO:YHDD

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PomBase:SPCC1020.10

F

Seeded From UniProt

complete

SCHPO:YHDD

involved_in

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • has_input:(PomBase:SPAP7G5.06)

Seeded From UniProt

complete

SCHPO:YHDD

involved_in

GO:0006808: regulation of nitrogen utilization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:YI26

involved_in

GO:0019740: nitrogen utilization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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