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PMID:20399744

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Citation

Jung, SY and Ko, YG (2010) TRIM72, a novel negative feedback regulator of myogenesis, is transcriptionally activated by the synergism of MyoD (or myogenin) and MEF2. Biochem. Biophys. Res. Commun. 396:238-45

Abstract

TRIM72 is known to be involved in the negative feedback regulation of myogenesis by targeting insulin receptor substrate-1. Here, we found that TRIM72 was more highly expressed in oxidative muscle with the higher activity of MEF2, compared to glycolytic muscle. Indeed, TRIM72 promoter contained an evolutionarily conserved MEF2 site juxtaposed to E-box. TRIM72 promoter activity was decreased by the site-directed mutagenesis of either E-boxes or a MEF2 site and synergistically enhanced by MyoD (or myogenin) and MEF2, which were associated with proximal E-box, and MEF2 site of the TRIM72 promoter, respectively. Taken together all these data, we concluded that the synergism of MyoD (or myogenin) and MEF2 is necessary for TRIM72 expression during C2C12 differentiation.

Links

PubMed Online version:10.1016/j.bbrc.2010.04.072

Keywords

Animals; Carrier Proteins/genetics; Cell Line; Chromatin Immunoprecipitation; E-Box Elements; Feedback, Physiological; Mice; Mice, Inbred C57BL; Muscle Development/genetics; MyoD Protein/genetics; MyoD Protein/metabolism; Myogenic Regulatory Factors/metabolism; Promoter Regions, Genetic; Transcription, Genetic; Transcriptional Activation

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:MYOG

enables

GO:0000987: cis-regulatory region sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:MYOG

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:99458

P

Seeded From UniProt

complete

MOUSE:MYOG

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:99532

P

Seeded From UniProt

complete

MOUSE:MYOG

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:99533

P

Seeded From UniProt

complete

MOUSE:MEF2A

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:97276

P

Seeded From UniProt

complete

MOUSE:MEF2D

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:97276

P

Seeded From UniProt

complete

MOUSE:MEF2C

enables

GO:0000987: cis-regulatory region sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:MEF2C

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:97276

P

Seeded From UniProt

complete

MOUSE:MEF2C

acts_upstream_of_or_within

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:97276

P

Seeded From UniProt

complete

MOUSE:MEF2C

enables

GO:0000987: proximal promoter sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

has_participant:(MGI:MGI:3612190)

Seeded From UniProt

complete


See also

References

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