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PMID:20398064

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Citation

Murata, Y, Mori, M, Kotani, T, Supriatna, Y, Okazawa, H, Kusakari, S, Saito, Y, Ohnishi, H and Matozaki, T (2010) Tyrosine phosphorylation of R3 subtype receptor-type protein tyrosine phosphatases and their complex formations with Grb2 or Fyn. Genes Cells 15:513-24

Abstract

Post-translational modification of protein tyrosine phosphatases (PTPs) is implicated in functional modulation of these enzymes. Stomach cancer-associated protein tyrosine phosphatase-1 (SAP-1), as well as protein tyrosine phosphatase receptor type O (PTPRO) and vascular endothelial-protein tyrosine phosphatase (VE-PTP) are receptor-type PTPs (RPTPs), which belong to the R3 subtype RPTP family. Here, we have shown that the carboxyl (COOH)-terminal region of SAP-1 undergoes tyrosine phosphorylation by the treatment with a PTP inhibitor. Src family kinases are important for the tyrosine phosphorylation of SAP-1. Either Grb2 or Fyn, through their Src homology-2 domains, bound to the tyrosine-phosphorylated SAP-1. Moreover, both PTPRO and VE-PTP underwent tyrosine phosphorylation in their COOH-terminal regions. Tyrosine phosphorylation of VE-PTP or PTPRO also promoted their complex formations with Grb2 or Fyn. Forced expression of SAP-1, PTPRO or VE-PTP promoted cell spreading and lamellipodium formation of fibroblasts that expressed an activated form of Ras. In contrast, such effects of non-tyrosine-phosphorylated forms of these RPTPs were markedly smaller than those of wild-type RPTPs. Our results thus suggest that tyrosine phosphorylation of R3 subtype RPTPs promotes their complex formations with Grb2 or Fyn and thus participates in the regulation of cell morphology.

Links

PubMed Online version:10.1111/j.1365-2443.2010.01398.x

Keywords

Amino Acid Motifs; Animals; Cell Line; GRB2 Adaptor Protein/genetics; GRB2 Adaptor Protein/metabolism; Humans; Mice; Mice, Inbred C57BL; Phosphorylation; Proto-Oncogene Proteins c-fyn/genetics; Proto-Oncogene Proteins c-fyn/metabolism; Pseudopodia/metabolism; Receptor-Like Protein Tyrosine Phosphatases, Class 3/genetics; Receptor-Like Protein Tyrosine Phosphatases, Class 3/metabolism; Tyrosine/genetics; Tyrosine/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:PTPRO

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q60631

F

Seeded From UniProt

complete

MOUSE:GRB2

enables

GO:0019903: protein phosphatase binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:E9Q612

F

Seeded From UniProt

complete

MOUSE:PTPRO

involved_in

GO:0000902: cell morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:PTPRO

involved_in

GO:0030032: lamellipodium assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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