GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:20395473

From GONUTS
Jump to: navigation, search
Citation

Samara, NL, Datta, AB, Berndsen, CE, Zhang, X, Yao, T, Cohen, RE and Wolberger, C (2010) Structural insights into the assembly and function of the SAGA deubiquitinating module. Science 328:1025-9

Abstract

SAGA is a transcriptional coactivator complex that is conserved across eukaryotes and performs multiple functions during transcriptional activation and elongation. One role is deubiquitination of histone H2B, and this activity resides in a distinct subcomplex called the deubiquitinating module (DUBm), which contains the ubiquitin-specific protease Ubp8, bound to Sgf11, Sus1, and Sgf73. The deubiquitinating activity depends on the presence of all four DUBm proteins. We report here the 1.90 angstrom resolution crystal structure of the DUBm bound to ubiquitin aldehyde, as well as the 2.45 angstrom resolution structure of the uncomplexed DUBm. The structure reveals an arrangement of protein domains that gives rise to a highly interconnected complex, which is stabilized by eight structural zinc atoms that are critical for enzymatic activity. The structure suggests a model for how interactions with the other DUBm proteins activate Ubp8 and allows us to speculate about how the DUBm binds to monoubiquitinated histone H2B in nucleosomes.

Links

PubMed Online version:10.1126/science.1190049

Keywords

Aldehydes/chemistry; Aldehydes/metabolism; Crystallography, X-Ray; Endopeptidases/chemistry; Endopeptidases/metabolism; Histone Acetyltransferases/chemistry; Histone Acetyltransferases/metabolism; Histones/metabolism; Models, Biological; Models, Molecular; Nuclear Proteins/chemistry; Nuclear Proteins/metabolism; Nucleosomes/chemistry; Nucleosomes/metabolism; Protein Binding; Protein Conformation; Protein Structure, Tertiary; RNA-Binding Proteins/chemistry; RNA-Binding Proteins/metabolism; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/metabolism; Trans-Activators/chemistry; Trans-Activators/metabolism; Transcription Factors/chemistry; Transcription Factors/metabolism; Ubiquitin/chemistry; Ubiquitin/metabolism; Ubiquitinated Proteins/metabolism; Ubiquitination; Ubiquitins/chemistry; Ubiquitins/metabolism; Zinc/chemistry; Zinc/metabolism; Zinc Fingers

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:UBP8

part_of

GO:0071819: DUBm complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:SGF73

part_of

GO:0071819: DUBm complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:SGF11

part_of

GO:0071819: DUBm complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:SUS1

part_of

GO:0071819: DUBm complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.