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PMID:20395451

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Citation

Lee, SJ, Kang, JY, Park, HJ, Kim, MD, Bae, MS, Choi, HI and Kim, SY (2010) DREB2C interacts with ABF2, a bZIP protein regulating abscisic acid-responsive gene expression, and its overexpression affects abscisic acid sensitivity. Plant Physiol. 153:716-27

Abstract

ABF2 is a basic leucine zipper protein that regulates abscisic acid (ABA)-dependent stress-responsive gene expression. We carried out yeast two-hybrid screens to isolate genes encoding ABF2-interacting proteins in Arabidopsis (Arabidopsis thaliana). Analysis of the resulting positive clones revealed that two of them encode an AP2 domain protein, which is the same as AtERF48/DREB2C. This protein, which will be referred to as DREB2C, could bind C-repeat/dehydration response element in vitro and possesses transcriptional activity. To determine its function, we generated DREB2C overexpression lines and investigated their phenotypes. The transgenic plants were ABA hypersensitive during germination and seedling establishment stages, whereas primary root elongation of seedlings was ABA insensitive, suggesting developmental stage dependence of DREB2C function. The DREB2C overexpression lines also displayed altered stress response; whereas the plants were dehydration sensitive, they were freezing and heat tolerant. We further show that other AP2 domain proteins, DREB1A and DREB2A, interact with ABF2 and that other ABF family members, ABF3 and ABF4, interact with DREB2C. Previously, others demonstrated that ABF and DREB family members cooperate to activate the transcription of an ABA-responsive gene. Our result implies that the cooperation of the two classes of transcription factors may involve physical interaction.

Links

PubMed PMC2879808 Online version:10.1104/pp.110.154617

Keywords

Abscisic Acid/metabolism; Amino Acid Sequence; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Basic-Leucine Zipper Transcription Factors/genetics; Basic-Leucine Zipper Transcription Factors/metabolism; Cold Temperature; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Dehydration; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Germination; Hot Temperature; Molecular Sequence Data; Plant Roots/growth & development; Plants, Genetically Modified/genetics; Plants, Genetically Modified/metabolism; RNA, Plant/genetics; Seedling/growth & development; Stress, Physiological; Transcription Factors/genetics; Transcription Factors/metabolism; Two-Hybrid System Techniques

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:DRE2C

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

AGI_LocusCode:AT1G45249

F

Seeded From UniProt

complete

ARATH:DRE2C

acts_upstream_of_or_within

GO:0009737: response to abscisic acid

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DRE2C

enables

GO:0003700: DNA-binding transcription factor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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