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PMID:20388707

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Citation

Yi, Y, Kamata-Sakurai, M, Denda-Nagai, K, Itoh, T, Okada, K, Ishii-Schrade, K, Iguchi, A, Sugiura, D and Irimura, T (2010) Mucin 21/epiglycanin modulates cell adhesion. J. Biol. Chem. 285:21233-40

Abstract

The molecular structure of mouse Mucin 21 (Muc21)/epiglycanin is proposed to have 98 tandem repeats of 15 amino acids and three exceptional repeats with 12 or 13 amino acids each, followed by a stem domain, a transmembrane domain, and a cytoplasmic tail. A cDNA of Muc21 having 84 tandem repeats of 15 amino acids was constructed and transfected using a Venus vector into HEK 293T cells. The fluorescent cells, which were considered to express Muc21, were nonadherent. This antiadhesion effect was lessened when constructs with smaller numbers of tandem repeats were used, suggesting that the tandem repeat domain plays a crucial role. Cells expressing Muc21 were significantly less adherent to each other and to extracellular matrix components than control cells. Antibody binding to the cell surface integrin subunits alpha5, alpha6, and beta1 was reduced in Muc21 transfectants in a tandem repeat-dependent manner, whereas equal amounts of proteins were detected by Western blot analysis. Muc21 was expressed as a large glycoprotein that was highly glycosylated with O-glycans at the cell surface, as detected by flow cytometry, Western blotting, and lectin blotting. Although at least a portion of Muc21 was glycosylated with sialylated glycans, removal of sialic acid did not influence the prevention of adhesion.

Links

PubMed PMC2898422 Online version:10.1074/jbc.M109.082875

Keywords

Amino Acids/chemistry; Animals; Cell Adhesion; Cell Line; Cell Line, Tumor; Gene Expression Regulation; Glycoproteins/chemistry; Glycosylation; Humans; Membrane Glycoproteins/metabolism; Mice; Models, Biological; Mucins/metabolism; Polysaccharides/chemistry; Protein Structure, Tertiary

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:A8QW50

acts_upstream_of_or_within

GO:0022408: negative regulation of cell-cell adhesion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:A8QW50

acts_upstream_of_or_within

GO:0010812: negative regulation of cell-substrate adhesion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:A8QW50

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:D9N008

acts_upstream_of_or_within

GO:0022408: negative regulation of cell-cell adhesion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:D9N008

acts_upstream_of_or_within

GO:0010812: negative regulation of cell-substrate adhesion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:D9N008

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:F7C950

acts_upstream_of_or_within

GO:0022408: negative regulation of cell-cell adhesion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:F7C950

acts_upstream_of_or_within

GO:0010812: negative regulation of cell-substrate adhesion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:F7C950

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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