GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:20385102

From GONUTS
Jump to: navigation, search
Citation

Zaidel-Bar, R, Miller, S, Kaminsky, R and Broday, L (2010) Molting-specific downregulation of C. elegans body-wall muscle attachment sites: the role of RNF-5 E3 ligase. Biochem. Biophys. Res. Commun. 395:509-14

Abstract

Repeated molting of the cuticula is an integral part of arthropod and nematode development. Shedding of the old cuticle takes place on the surface of hypodermal cells, which are also responsible for secretion and synthesis of a new cuticle. Here, we use the model nematode Caenorhabditis elegans to show that muscle cells, laying beneath and mechanically linked to the hypodermis, play an important role during molting. We followed the molecular composition and distribution of integrin mediated adhesion structures called dense bodies (DB), which indirectly connect muscles to the hypodermis. We found the concentration of two DB proteins (PAT-3/beta-integrin and UNC-95) to decrease during the quiescent phase of molting, concomitant with an apparent increase in lateral movement of the DB. We show that levels of the E3-ligase RNF-5 increase specifically during molting, and that RNF-5 acts to ubiquitinate the DB protein UNC-95. Persistent high levels of RNF-5 driven by a heatshock or unc-95 promoter lead to failure of ecdysis, and in non-molting worms to a progressive detachment of the cuticle from the hypodermis. These observations indicate that increased DB dynamics characterizes the lethargus phase of molting in parallel to decreased levels of DB components and that temporal expression of RNF-5 contributes to an efficient molting process.

Links

PubMed Online version:10.1016/j.bbrc.2010.04.049

Keywords

Animals; Caenorhabditis elegans/enzymology; Caenorhabditis elegans/physiology; Caenorhabditis elegans Proteins/metabolism; Caenorhabditis elegans Proteins/physiology; Carrier Proteins/metabolism; Carrier Proteins/physiology; Down-Regulation; Integrin beta Chains/metabolism; Molting; Muscle, Skeletal/enzymology; Muscle, Skeletal/physiology; Ubiquitin-Protein Ligases/physiology; Ubiquitination

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:RNF5

involved_in

GO:0018996: molting cycle, collagen and cuticulin-based cuticle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:RNF5

involved_in

GO:0006511: ubiquitin-dependent protein catabolic process

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:RNF5

enables

GO:0061630: ubiquitin protein ligase activity

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_input:(UniProtKB:Q9NEZ5)

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.