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PMID:20375058

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Citation

Fujihara, Y, Murakami, M, Inoue, N, Satouh, Y, Kaseda, K, Ikawa, M and Okabe, M (2010) Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse. J. Cell. Sci. 123:1531-6

Abstract

Mammalian fertilization is a multistep process that culminates in the fusion of the sperm and egg plasma membrane. It is widely accepted that the equatorial segment of the acrosome-reacted sperm is important in initiating fusion with the egg plasma membrane during fertilization. There are various proteins known to be distributed only in the equatorial segment of sperm. The role of these proteins must be clarified to understand the membrane fusion process. We produced a mouse line that lacked SPESP1 (sperm equatorial segment protein 1) and analyzed the fertilizing ability of the sperm. The average number of pups that were fathered by Spesp1(+/-) and Spesp1(-/-) males was significantly lower than that of wild-type fathers. In these mouse lines, fewer sperm were found to migrate into oviducts and fewer eggs were fertilized. The Spesp1(+/-) and Spesp1(-/-) sperm showed a lower fusing ability compared with the wild-type sperm. The disruption of Spesp1 was shown to cause an aberrant distribution of various sperm proteins. Moreover, scanning electron microscopy revealed that the membrane in the equatorial segment area, which usually forms an acrosomal sheath, disappears after acrosome reaction in Spesp1-deficient mice. It was demonstrated that SPESP1 is necessary to produce the fully 'fusion competent' sperm.

Links

PubMed Online version:10.1242/jcs.067363

Keywords

Animals; Blotting, Western; Carrier Proteins/genetics; Carrier Proteins/metabolism; Fertilization/physiology; Gene Expression Regulation; Male; Membrane Proteins/metabolism; Mice; Microscopy, Electron; Protein Transport; Seminal Plasma Proteins/genetics; Seminal Plasma Proteins/metabolism; Spermatozoa/cytology; Spermatozoa/metabolism; Spermatozoa/ultrastructure

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:SPESP

located_in

GO:0001669: acrosomal vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:SPESP

involved_in

GO:0007340: acrosome reaction

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:4838302

P

Seeded From UniProt

complete

MOUSE:SPESP

involved_in

GO:0007342: fusion of sperm to egg plasma membrane involved in single fertilization

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:4838302

P

Seeded From UniProt

complete

MOUSE:IZUM1

located_in

GO:0002080: acrosomal membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:IZUM1

part_of

GO:0002080: acrosomal membrane

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(CL:0000018)

Seeded From UniProt

complete


See also

References

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