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PMID:20374532

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Citation

Baek, D, Pathange, P, Chung, JS, Jiang, J, Gao, L, Oikawa, A, Hirai, MY, Saito, K, Pare, PW and Shi, H (2010) A stress-inducible sulphotransferase sulphonates salicylic acid and confers pathogen resistance in Arabidopsis. Plant Cell Environ. 33:1383-92

Abstract

Sulphonation of small molecules by cytosolic sulphotransferases in mammals is an important process in which endogenous molecules are modified for inactivation/activation of their biological effects. Plants possess large numbers of sulphotransferase genes, but their biological functions are largely unknown. Here, we present a functional analysis of the Arabidopsis sulphotransferase AtSOT12 (At2g03760). AtSOT12 gene expression is strongly induced by salt, and osmotic stress and hormone treatments. The T-DNA knock-out mutant sot12 exhibited hypersensitivity to NaCl and ABA in seed germination, and to salicylic acid (SA) in seedling growth. In vitro enzyme activity assay revealed that AtSOT12 sulphonates SA, and endogenous SA levels suggested that sulphonation of SA positively regulates SA production. Upon challenging with the pathogen Pseudomonas syringae, sot12 mutant and AtSOT12 over-expressing lines accumulate less and more SA, respectively, when compared with wild type. Consistent with the changes in SA levels, the sot12 mutant was more susceptible, while AtSOT12 over-expressing plants are more resistant to pathogen infection. Moreover, pathogen-induced PR gene expression in systemic leaves was significantly enhanced in AtSOT12 over-expressing plants. The role of sulphonation of SA in SA production, mobile signalling and acquired systemic resistance is discussed.

Links

PubMed Online version:10.1111/j.1365-3040.2010.02156.x

Keywords

Abscisic Acid/pharmacology; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/microbiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Immunity, Innate; Mutagenesis, Insertional; Mutation; Plant Diseases/genetics; Pseudomonas syringae; Salicylic Acid/metabolism; Sodium Chloride/pharmacology; Sulfotransferases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:SOT12

enables

GO:0008146: sulfotransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:SOT12

acts_upstream_of_or_within

GO:0006952: defense response

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SOT12

acts_upstream_of_or_within

GO:0009751: response to salicylic acid

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SOT12

acts_upstream_of_or_within

GO:0009651: response to salt stress

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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