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PMID:20367464

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Citation

Chen, L, Zhang, L and Yu, D (2010) Wounding-induced WRKY8 is involved in basal defense in Arabidopsis. Mol. Plant Microbe Interact. 23:558-65

Abstract

The WRKY family of plant transcription factors controls several types of plant stress responses. Arabidopsis WRKY8, localized to the nucleus, is mainly induced by abscissic acid, H(2)O(2), wounding, Pseudomonas syringae and Botrytis cinerea infection, and aphid and maggot feeding. To determine its biological functions, we isolated loss-of-function T-DNA insertion mutants and generated gain-of-function overexpressing WRKY8 transgenic plants in Arabidopsis. Plants expressing the mutated WRKY8 gene showed increased resistance to P. syringae but slightly decreased resistance to B. cinerea. In contrast, transgenic plants overexpressing WRKY8 were more susceptible to P. syringae infection but more resistant to B. cinerea infection. The contrasting responses to the two pathogens were correlated with opposite effects on pathogen-induced expression of two genes; salicylic acid-regulated PATHOGENESIS-RELATED1 (PR1) and jasmonic acid-regulated PDF1.2. Therefore, our results suggest that WRKY8 is a negative regulator of basal resistance to P. syringae and positive regulator to B. cinerea.

Links

PubMed Online version:10.1094/MPMI-23-5-0558

Keywords

Amino Acid Sequence; Arabidopsis/cytology; Arabidopsis/genetics; Arabidopsis/immunology; Arabidopsis/microbiology; Arabidopsis Proteins/biosynthesis; Arabidopsis Proteins/chemistry; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Botrytis/physiology; DNA, Bacterial/genetics; Gene Expression Regulation, Plant; Glucuronidase/metabolism; Molecular Sequence Data; Mutagenesis, Insertional/genetics; Mutation/genetics; Plant Diseases/genetics; Plant Diseases/immunology; Protein Transport; Pseudomonas syringae/physiology; Sequence Alignment; Signal Transduction; Subcellular Fractions/metabolism; Transcription Factors/biosynthesis; Transcription Factors/chemistry; Transcription Factors/genetics; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:WRKY8

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:WRKY8

acts_upstream_of_or_within

GO:0009737: response to abscisic acid

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:WRKY8

acts_upstream_of_or_within

GO:0050832: defense response to fungus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:WRKY8

acts_upstream_of_or_within

GO:0070301: cellular response to hydrogen peroxide

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:WRKY8

acts_upstream_of_or_within

GO:0042742: defense response to bacterium

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:WRKY8

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:WRKY8

involved_in

GO:0050832: defense response to fungus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:WRKY8

involved_in

GO:0042742: defense response to bacterium

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:WRKY8

involved_in

GO:0009737: response to abscisic acid

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:WRKY8

involved_in

GO:0070301: cellular response to hydrogen peroxide

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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