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PMID:20357107

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Citation

Yao, CA and Carlson, JR (2010) Role of G-proteins in odor-sensing and CO2-sensing neurons in Drosophila. J. Neurosci. 30:4562-72

Abstract

A central question in insect chemoreception is whether signaling occurs via G-proteins. Two families of seven-transmembrane-domain chemoreceptors, the odor (Or) and gustatory receptor (Gr) families, have been identified in Drosophila (Clyne et al., 1999, 2000; Vosshall et al., 1999). Ors mediate odor responses, whereas two Grs, Gr21a and Gr63a, mediate CO2 response (Hallem et al., 2004; Jones et al., 2007; Kwon et al., 2007). Using single-sensillum recordings, we systematically investigate the role of Galpha proteins in vivo, initially with RNA interference constructs, competitive peptides, and constitutively active Galpha proteins. The results do not support a role for Galpha proteins in odor sensitivity. In parallel experiments, manipulations of Galpha(q), but not other Galpha proteins, affected CO2 response. Transient, conditional, and ectopic expression analyses consistently supported a role for Galpha(q) in the response of CO2-sensing neurons, but not odor-sensing neurons. Genetic mosaic analysis confirmed that odor responses are normal in the absence of Galpha(q). Ggamma30A is also required for normal CO2 response. The simplest interpretation of these results is that Galpha(q) and Ggamma30A play a role in the response of CO2-sensing neurons, but are not required for Or-mediated odor signaling.

Links

PubMed PMC2858456 Online version:10.1523/JNEUROSCI.6357-09.2010

Keywords

Animals; Carbon Dioxide/physiology; Chemoreceptor Cells/physiology; Drosophila/physiology; GTP-Binding Protein alpha Subunits/genetics; GTP-Binding Protein alpha Subunits/physiology; GTP-Binding Protein alpha Subunits, Gi-Go/genetics; GTP-Binding Protein alpha Subunits, Gi-Go/physiology; GTP-Binding Protein alpha Subunits, Gq-G11/genetics; GTP-Binding Protein alpha Subunits, Gq-G11/physiology; GTP-Binding Protein alpha Subunits, Gs/genetics; GTP-Binding Protein alpha Subunits, Gs/physiology; GTP-Binding Protein gamma Subunits/genetics; GTP-Binding Protein gamma Subunits/physiology; Mutation; Odors; Olfactory Pathways; Smell

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:GNAQ

involved_in

GO:0071244: cellular response to carbon dioxide

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:GBGE

involved_in

GO:0071244: cellular response to carbon dioxide

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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