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PMID:20356456

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Citation

Kim, JS, Bang, MA, Lee, S, Chae, HZ and Kim, K (2010) Distinct functional roles of peroxiredoxin isozymes and glutathione peroxidase from fission yeast, Schizosaccharomyces pombe. BMB Rep 43:170-5

Abstract

To investigate the differences in the functional roles of peroxiredoxins (Prxs) and glutathione peroxidase (GPx) of Schizosaccharomyces pombe, we examined the peroxidase and molecular chaperone properties of the recombinant proteins. TPx (thioredoxin peroxidase) exhibited a capacity for peroxide reduction with the thioredoxin system. GPx also showed thioreoxin-dependent peroxidase activity rather than GPx activity. The peroxidase activity of BCP (bacterioferritin comigratory protein) was similar to that of TPx. However, peroxidase activity was not observed for PMP20 (peroxisomal membrane protein 20). TPx, PMP20, and GPx inhibited thermal aggregation of citrate synthase at 43(o)C, but BCP failed to inhibit the aggregation. The chaperone activities of PMP20 and GPx were weaker than that of TPx. The peroxidase and chaperone properties of TPx, BCP, and GPx of the fission yeast are similar to those of Saccharomyces cerevisiae. The fission yeast PMP20 without thioredoxin-dependent peroxidase activity may act as a molecular chaperone.

Links

PubMed

Keywords

Citrate (si)-Synthase/metabolism; Glutathione Peroxidase/genetics; Glutathione Peroxidase/metabolism; Glutathione Peroxidase/physiology; Isoenzymes/genetics; Isoenzymes/metabolism; Isoenzymes/physiology; Peroxiredoxins/genetics; Peroxiredoxins/metabolism; Peroxiredoxins/physiology; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Schizosaccharomyces/enzymology; Schizosaccharomyces pombe Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

SCHPO:PMP20

enables

GO:0051082: unfolded protein binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:PMP20

NOT|enables

GO:0008379: thioredoxin peroxidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:PMP20

NOT|enables

GO:0004602: glutathione peroxidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:PMP20

involved_in

GO:0042026: protein refolding

ECO:0000305: curator inference used in manual assertion

GO:0051082

P

Seeded From UniProt

complete

SCHPO:GPX1

NOT|enables

GO:0008379: thioredoxin peroxidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:GPX1

NOT|enables

GO:0004602: glutathione peroxidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:TPX1

enables

GO:0051082: unfolded protein binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:TPX1

enables

GO:0008379: thioredoxin peroxidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:PRX

enables

GO:0008379: thioredoxin peroxidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:PRX

involved_in

GO:0019430: removal of superoxide radicals

ECO:0000305: curator inference used in manual assertion

GO:0008379

P

Seeded From UniProt

complete

SCHPO:PRX

involved_in

GO:0042744: hydrogen peroxide catabolic process

ECO:0000305: curator inference used in manual assertion

GO:0008379

P

Seeded From UniProt

complete

SCHPO:TSA1

enables

GO:0008379: thioredoxin peroxidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:TSA1

enables

GO:0051082: unfolded protein binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

Notes

See also

References

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