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PMID:20348213

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Citation

Hurley, BA, Tran, HT, Marty, NJ, Park, J, Snedden, WA, Mullen, RT and Plaxton, WC (2010) The dual-targeted purple acid phosphatase isozyme AtPAP26 is essential for efficient acclimation of Arabidopsis to nutritional phosphate deprivation. Plant Physiol. 153:1112-22

Abstract

Induction of intracellular and secreted acid phosphatases (APases) is a widespread response of orthophosphate (Pi)-starved (-Pi) plants. APases catalyze Pi hydrolysis from a broad range of phosphomonoesters at an acidic pH. The largest class of nonspecific plant APases is comprised of the purple APases (PAPs). Although the biochemical properties, subcellular location, and expression of several plant PAPs have been described, their physiological functions have not been fully resolved. Recent biochemical studies indicated that AtPAP26, one of 29 PAPs encoded by the Arabidopsis (Arabidopsis thaliana) genome, is the predominant intracellular APase, as well as a major secreted APase isozyme up-regulated by -Pi Arabidopsis. An atpap26 T-DNA insertion mutant lacking AtPAP26 transcripts and 55-kD immunoreactive AtPAP26 polypeptides exhibited: (1) 9- and 5-fold lower shoot and root APase activity, respectively, which did not change in response to Pi starvation, (2) a 40% decrease in secreted APase activity during Pi deprivation, (3) 35% and 50% reductions in free and total Pi concentration, respectively, as well as 5-fold higher anthocyanin levels in shoots of soil-grown -Pi plants, and (4) impaired shoot and root development when subjected to Pi deficiency. By contrast, no deleterious influence of AtPAP26 loss of function occurred under Pi-replete conditions, or during nitrogen or potassium-limited growth, or oxidative stress. Transient expression of AtPAP26-mCherry in Arabidopsis suspension cells verified that AtPAP26 is targeted to the cell vacuole. Our results confirm that AtPAP26 is a principal contributor to Pi stress-inducible APase activity, and that it plays an important role in the Pi metabolism of -Pi Arabidopsis.

Links

PubMed PMC2899917 Online version:10.1104/pp.110.153270

Keywords

Acclimatization/drug effects; Acid Phosphatase/genetics; Acid Phosphatase/metabolism; Acid Phosphatase/secretion; Alleles; Arabidopsis/cytology; Arabidopsis/drug effects; Arabidopsis/enzymology; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Arabidopsis Proteins/secretion; DNA, Bacterial/genetics; Gene Expression Regulation, Enzymologic/drug effects; Gene Expression Regulation, Plant/drug effects; Glycoproteins/genetics; Glycoproteins/metabolism; Glycoproteins/secretion; Intracellular Space/drug effects; Intracellular Space/enzymology; Isoenzymes/metabolism; Isoenzymes/secretion; Mutagenesis, Insertional/drug effects; Mutagenesis, Insertional/genetics; Mutation/genetics; Phosphates/deficiency; Phosphates/pharmacology; Protein Transport/drug effects; Reproducibility of Results; Seedling/drug effects; Seedling/enzymology; Seedling/growth & development; Up-Regulation/drug effects; Vacuoles/drug effects; Vacuoles/enzymology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:PPA26

located_in

GO:0005773: vacuole

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PPA26

acts_upstream_of_or_within

GO:0055062: phosphate ion homeostasis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:PPA26

part_of

GO:0005773: vacuole

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:PPA26

involved_in

GO:0055062: phosphate ion homeostasis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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