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PMID:20348034

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Citation

Atzei, P, Yang, F, Collery, R, Kennedy, BN and Moynagh, PN (2010) Characterisation of expression patterns and functional role of Cactin in early zebrafish development. Gene Expr. Patterns 10:199-206

Abstract

The immune system of teleost zebrafish (Danio rerio) shows high similarity to mammalian counterparts sharing many innate immune components including Toll-Like Receptors (TLRs), cytokines, chemokines and complement molecules. As in mammals, zebrafish also contains the transcription factor NF-kappaB that plays dualist roles in innate immunity and early development. Indeed NF-kappaB members are expressed in different temporal patterns during the early stages of zebrafish embryogenesis indicating that each molecule is involved in specific developmental events. In the present study we employ zebrafish as a model to characterise the expression pattern and role of a novel NF-kappaB regulator, termed Cactin, in early development. Cactin was first characterised in Drosophila as a new member of the Rel pathway that could affect the generation of dorsal-ventral polarity. To explore the potential developmental role of Cactin in zebrafish, we initially investigated its expression pattern and functional role during early embryonic developmental stages. We detect Cactin expression at all stages of early development and knockdown of Cactin by specific morpholino antisense oligonucleotides causes developmental abnormalities manifested by an overall dysmorphic cellular organisation. These results indicate that Cactin has been highly conserved during evolution and plays a key role in early embryonic development.

Links

PubMed Online version:10.1016/j.gep.2010.03.003

Keywords

Animals; Base Sequence; Carrier Proteins/genetics; DNA Primers; Drosophila Proteins/genetics; Gene Expression Regulation, Developmental; Gene Knockdown Techniques; Humans; Polymerase Chain Reaction; Zebrafish/embryology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:CATIN

involved_in

GO:0003407: neural retina development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-100726-2

P

Seeded From UniProt

complete

DANRE:CATIN

involved_in

GO:0001654: eye development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:CATIN

involved_in

GO:0008595: anterior/posterior axis specification, embryo

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:CATIN

involved_in

GO:0040019: positive regulation of embryonic development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:E9QDZ9

GO:0040019: positive regulation of embryonic development

ECO:0000315:

P

Fig 3

complete
CACAO 7265

DANRE:E9QDZ9

involved_in

GO:0040019: positive regulation of embryonic development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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