GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:20339383

From GONUTS
Jump to: navigation, search
Citation

Kogo, H, Kowa-Sugiyama, H, Yamada, K, Bolor, H, Tsutsumi, M, Ohye, T, Inagaki, H, Taniguchi, M, Toda, T and Kurahashi, H (2010) Screening of genes involved in chromosome segregation during meiosis I: toward the identification of genes responsible for infertility in humans. J. Hum. Genet. 55:293-9

Abstract

Prophase I of male meiosis during early spermatogenesis involves dynamic chromosome segregation processes, including synapsis, meiotic recombination and cohesion. Genetic defects in the genes that participate in these processes consistently cause reproduction failure in mice. To identify candidate genes responsible for infertility in humans, we performed gene expression profiling of mouse spermatogenic cells undergoing meiotic prophase I. Cell fractions enriched in spermatogonia, leptotene/zygotene spermatocytes or pachytene spermatocytes from developing mouse testis were separately isolated by density gradient sedimentation and subjected to microarray analysis. A total of 726 genes were identified that were upregulated in leptotene/zygotene spermatocytes. To evaluate the screening efficiency for meiosis-specific genes, we randomly selected 12 genes from this gene set and characterized each gene product using reverse transcription (RT)-PCR of RNA from gonadal tissues, in situ hybridization on testicular tissue sections and subcellular localization analysis of the encoded protein. Four of the 12 genes were confirmed as genes expressed in meiotic stage and 2 of these 4 genes were novel, previously uncharacterized genes. Among the three confirmation methods that were used, RT-PCR appeared to be the most efficient method for further screening. These 726 candidates for human infertility genes might serve as a useful resource for next-generation sequencing combined with exon capture by microarray.

Links

PubMed Online version:10.1038/jhg.2010.26

Keywords

Animals; Cell Line; Chromosome Segregation/genetics; Endodeoxyribonucleases; Esterases/genetics; Esterases/metabolism; Female; Fluorescent Antibody Technique; Gene Expression Profiling; Genetic Predisposition to Disease/genetics; Humans; In Situ Hybridization; Infertility, Male/genetics; Male; Meiotic Prophase I/genetics; Mice; Mice, Inbred C57BL; Oligonucleotide Array Sequence Analysis; Reverse Transcriptase Polymerase Chain Reaction; Spermatocytes/cytology; Spermatocytes/metabolism; Testis/cytology; Testis/metabolism; Transfection

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:SPDYA

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:SPDYA

involved_in

GO:0007140: male meiotic nuclear division

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:SMC5

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:CTSRZ

involved_in

GO:0007140: male meiotic nuclear division

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:MEIOB

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:MEIOB

involved_in

GO:0007140: male meiotic nuclear division

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.