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PMID:20299458

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Citation

Zhang, Y, Smith, AD 4th, Renfrow, MB and Schneider, DA (2010) The RNA polymerase-associated factor 1 complex (Paf1C) directly increases the elongation rate of RNA polymerase I and is required for efficient regulation of rRNA synthesis. J. Biol. Chem. 285:14152-9

Abstract

The rate of ribosome synthesis is proportional to the rate of cell proliferation; thus, transcription of rRNA by RNA polymerase I (Pol I) is an important target for the regulation of this process. Most previous investigations into mechanisms that regulate the rate of ribosome synthesis have focused on the initiation step of transcription by Pol I; however, recent studies in yeast and mammals have identified factors that influence transcription elongation by Pol I. The RNA polymerase-associated factor 1 complex (Paf1C) is a transcription elongation factor with known roles in Pol II transcription. We previously identified a role for Paf1C in transcription elongation by Pol I. In this study, genetic interactions between genes for Paf1C and Pol I subunits confirm this conclusion. In vitro studies demonstrate that purified Paf1C directly increases the rate of transcription elongation by Pol I. Finally, we show that Paf1C function is required for efficient control of Pol I transcription in response to target of rapamycin (TOR) signaling or amino acid limitation. These studies demonstrate that Paf1C plays an important direct role in cellular control of rRNA expression.

Links

PubMed PMC2863250 Online version:10.1074/jbc.M110.115220

Keywords

Gene Expression Regulation, Fungal; Mass Spectrometry; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Promoter Regions, Genetic; RNA Polymerase I/genetics; RNA Polymerase I/metabolism; RNA, Ribosomal/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:LEO1

involved_in

GO:2001209: positive regulation of transcription elongation from RNA polymerase I promoter

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:RTF1

involved_in

GO:2001209: positive regulation of transcription elongation from RNA polymerase I promoter

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:CTR9

involved_in

GO:0006360: transcription by RNA polymerase I

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000005192

P

Seeded From UniProt

complete

YEAST:CTR9

involved_in

GO:2001209: positive regulation of transcription elongation from RNA polymerase I promoter

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:CDC73

involved_in

GO:2001209: positive regulation of transcription elongation from RNA polymerase I promoter

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:PAF1

involved_in

GO:0006360: transcription by RNA polymerase I

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:PAF1

involved_in

GO:0006360: transcription by RNA polymerase I

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000005192

P

Seeded From UniProt

complete

YEAST:PAF1

involved_in

GO:0006364: rRNA processing

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:PAF1

involved_in

GO:2001209: positive regulation of transcription elongation from RNA polymerase I promoter

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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