PMID:20237820

Citation	Hur, SK, Park, EJ, Han, JE, Kim, YA, Kim, JD, Kang, D and Kwon, J (2010) Roles of human INO80 chromatin remodeling enzyme in DNA replication and chromosome segregation suppress genome instability. Cell. Mol. Life Sci. 67:2283-96
Abstract	Although INO80 chromatin remodeling enzyme has been shown in yeast to play roles in non-transcriptional nuclear processes such as DNA replication, its cellular functions in higher eukaryotes have remained largely unexplored. Here, we provide evidence that human INO80 (hINO80) participates in both DNA replication and chromosome segregation during the normal cell division cycle. hINO80 binds to chromatin localizing at replication forks during the S-phase, and is required for efficient DNA synthesis and S-phase progression. Unexpectedly, hINO80 associates with spindle microtubule during mitosis, and its deficiency leads to defective microtubule assembly and abnormal chromosome segregation. Consistent with these results, hINO80 is critical for suppressing aneuploidy and structural chromosome abnormalities. This work therefore not only emphasizes the evolutionary importance of INO80 in DNA replication but also reveals a new role for this remodeler in chromosome segregation, both of which likely come into play in maintaining the genome integrity.
Links	PubMed Online version:10.1007/s00018-010-0337-3
Keywords	Aneuploidy; Chromatin/metabolism; Chromatin Assembly and Disassembly; Chromosome Segregation; DNA Helicases/metabolism; DNA Helicases/physiology; DNA Replication; Genomic Instability; HeLa Cells; Humans; Mitosis; S Phase
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:INO80	involved_in	GO:0000070: mitotic sister chromatid segregation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
HUMAN:INO80	involved_in	GO:0010571: positive regulation of nuclear cell cycle DNA replication	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
HUMAN:INO80	involved_in	GO:0030307: positive regulation of cell growth	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
HUMAN:INO80	enables	GO:0043014: alpha-tubulin binding	ECO:0000315: mutant phenotype evidence used in manual assertion		F		Seeded From UniProt	complete
HUMAN:INO80	involved_in	GO:0051225: spindle assembly	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
HUMAN:INO80	involved_in	GO:2000045: regulation of G1/S transition of mitotic cell cycle	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.