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PMID:20229090

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Citation

Kim, TH, Kim, do H, Nam, HW, Park, SY, Shim, J and Cho, JW (2010) Tyrosylprotein sulfotransferase regulates collagen secretion in Caenorhabditis elegans. Mol. Cells 29:413-8

Abstract

The sulfation of tyrosine residues is an important post-translational modification involved in the regulation of protein function. We examined the activity of worm tyrosylprotein sulfotransferase (TPST-1) on a typical cuticle collagen, ROL-6, in C. elegans. We verified that TPST-1 sulfates three tyrosine residues of ROL-6 in vitro. We found that these tyrosine residues are important for the secretion of ROL-6::GFP. Mutant ROL-6::GFP proteins that contain more than two substitutions of the target tyrosine residues are severely deficient in cuticle localization. Consistently, knock down of tpst-1 blocked the cuticle localization of ROL-6::GFP. Therefore, the sulfation of ROL-6 by TPST-1 is critical for the proper localization of ROL-6. We also confirmed that worm TPST-1 is localized to the trans-Golgi network (TGN). Our results indicate that TPST-1 regulates cuticle organization by promoting the transport of ROL-6 from the TGN to the cuticle.

Links

PubMed Online version:10.1007/s10059-010-0049-4

Keywords

Amino Acid Sequence; Animals; Blotting, Western; Caenorhabditis elegans/enzymology; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Caenorhabditis elegans Proteins/secretion; Cell Line; Collagen/genetics; Collagen/metabolism; Collagen/secretion; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; HeLa Cells; Humans; Microscopy, Fluorescence; Molecular Sequence Data; Mutation; Protein Transport; RNA Interference; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Sulfates/metabolism; Sulfotransferases/genetics; Sulfotransferases/metabolism; Transfection; Tyrosine/genetics; Tyrosine/metabolism; trans-Golgi Network/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:TPSTA

enables

GO:0008476: protein-tyrosine sulfotransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CAEEL:TPSTA

located_in

GO:0005802: trans-Golgi network

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:TPSTA

involved_in

GO:0006478: peptidyl-tyrosine sulfation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:ROL6

located_in

GO:0060102: collagen and cuticulin-based cuticle extracellular matrix

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:ROL6

enables

GO:0042329: structural constituent of collagen and cuticulin-based cuticle

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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